InnateDB Protein
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IDBP-163410.6
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Last Modified
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2014-10-13 [Report errors or provide feedback]
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Gene Symbol
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Paf1
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Protein Name
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Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae)
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Synonyms
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5730511K23Rik; AA675045;
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Species
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Mus musculus
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Ensembl Protein
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ENSMUSP00000003529
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InnateDB Gene
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IDBG-163408 (Paf1)
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Protein Structure
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Function |
Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys- 4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors (By similarity). {ECO:0000250}.
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Subcellular Localization |
Nucleus. Note=Punctuate distribution throughout the nucleus except in nucleoli and the perinuclear chromatin. {ECO:0000250}.
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Disease Associations |
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Tissue Specificity |
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Comments |
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Number of Interactions
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This gene and/or its encoded proteins are associated with 13 experimentally validated interaction(s) in this database.
They are also associated with 58 interaction(s) predicted by orthology.
Experimentally validated |
Total |
13
[view]
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Protein-Protein |
12
[view]
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Protein-DNA |
1
[view]
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Protein-RNA |
0
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DNA-DNA |
0
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RNA-RNA |
0
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DNA-RNA |
0
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Predicted by orthology |
Total |
58 [view]
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Molecular Function |
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Biological Process |
GO:0000122
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negative regulation of transcription from RNA polymerase II promoter
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GO:0001711
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endodermal cell fate commitment
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GO:0006351
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transcription, DNA-templated
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GO:0006378
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mRNA polyadenylation
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GO:0010390
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histone monoubiquitination
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GO:0016055
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Wnt signaling pathway
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GO:0019827
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stem cell maintenance
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GO:0031442
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positive regulation of mRNA 3'-end processing
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GO:0032968
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positive regulation of transcription elongation from RNA polymerase II promoter
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GO:0033523
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histone H2B ubiquitination
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GO:0045638
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negative regulation of myeloid cell differentiation
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GO:0045944
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positive regulation of transcription from RNA polymerase II promoter
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GO:0071222
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cellular response to lipopolysaccharide
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Cellular Component |
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PDB ID |
MGI:1923988
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InterPro |
IPR007133
RNA polymerase II-associated, Paf1
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PFAM |
PF03985
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PRINTS |
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PIRSF |
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SMART |
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TIGRFAMs |
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Modification |
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SwissProt |
Q8K2T8
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PhosphoSite |
PhosphoSite-Q8K2T8
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TrEMBL |
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UniProt Splice Variant |
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Entrez Gene |
54624
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UniGene |
Mm.7916
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RefSeq |
NP_062331
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MGI ID |
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MGI Symbol |
Paf1
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OMIM |
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CCDS |
CCDS21043
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HPRD |
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IMGT |
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EMBL |
AB041615
AK017762
AK134857
AK148538
BC029843
BC083337
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GenPept |
AAH29843
AAH83337
BAA95098
BAB30913
BAE22315
BAE28608
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