InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Xrcc5

Summary

InnateDB Protein

IDBP-165688.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Xrcc5

Protein Name

X-ray repair complementing defective repair in Chinese hamster cells 5

Synonyms

AI314015; Ku80; Ku86;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000027379

InnateDB Gene

IDBG-165686 (Xrcc5)

Protein Structure

UniProt Annotation

Function

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus. Nucleus, nucleolus {ECO:0000250}. Chromosome.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 9 experimentally validated interaction(s) in this database.
They are also associated with 159 interaction(s) predicted by orthology.

Experimentally validated
Total	9 [view]
Protein-Protein	5 [view]
Protein-DNA	2 [view]
Protein-RNA	0
DNA-DNA	2 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	159 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003684	damaged DNA binding
GO:0003690	double-stranded DNA binding
GO:0003691	double-stranded telomeric DNA binding
GO:0004003	ATP-dependent DNA helicase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008022	protein C-terminus binding
GO:0016817	hydrolase activity, acting on acid anhydrides
GO:0031625	ubiquitin protein ligase binding
GO:0044212	transcription regulatory region DNA binding
GO:0044822	poly(A) RNA binding
GO:0051575	5'-deoxyribose-5-phosphate lyase activity

Biological Process

GO:0000723	telomere maintenance
GO:0006302	double-strand break repair
GO:0006303	double-strand break repair via nonhomologous end joining
GO:0006310	DNA recombination
GO:0006351	transcription, DNA-templated
GO:0007420	brain development
GO:0008283	cell proliferation
GO:0032508	DNA duplex unwinding
GO:0042493	response to drug
GO:0045892	negative regulation of transcription, DNA-templated
GO:0050769	positive regulation of neurogenesis
GO:0060218	hematopoietic stem cell differentiation
GO:0071398	cellular response to fatty acid
GO:0071475	cellular hyperosmotic salinity response
GO:0071481	cellular response to X-ray

Cellular Component

GO:0000784	nuclear chromosome, telomeric region
GO:0005634	nucleus
GO:0005730	nucleolus
GO:0005737	cytoplasm
GO:0016020	membrane
GO:0043234	protein complex
GO:0043564	Ku70:Ku80 complex
GO:0070419	nonhomologous end joining complex

Protein Structure and Domains

PDB ID

MGI:104517

InterPro

IPR002035 von Willebrand factor, type A
IPR005160 Ku70/Ku80 C-terminal arm
IPR005161 Ku70/Ku80, N-terminal alpha/beta
IPR006164 Ku70/Ku80 beta-barrel domain
IPR014893 Ku, C-terminal
IPR016194 SPOC like C-terminal domain

PFAM

PF00092
PF03730
PF03731
PF02735
PF08785

PRINTS

PIRSF

SMART

SM00327
SM00559

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P27641