InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Aplf

Summary

InnateDB Protein

IDBP-165940.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Aplf

Protein Name

aprataxin and PNKP like factor

Synonyms

2010301N04Rik; AI452191;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000066232

InnateDB Gene

IDBG-165938 (Aplf)

Protein Structure

UniProt Annotation

Function

Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic- apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus. Cytoplasm, cytosol. Note=Localizes to DNA damage sites. Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 9 interaction(s) predicted by orthology.

Experimentally validated
Total	1 [view]
Protein-Protein	1 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	9 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0003906	DNA-(apurinic or apyrimidinic site) lyase activity
GO:0004520	endodeoxyribonuclease activity
GO:0005515	protein binding
GO:0008408	3'-5' exonuclease activity
GO:0046872	metal ion binding

Biological Process

GO:0000012	single strand break repair
GO:0000737	DNA catabolic process, endonucleolytic
GO:0006302	double-strand break repair
GO:0006974	cellular response to DNA damage stimulus
GO:0045191	regulation of isotype switching
GO:0090305	nucleic acid phosphodiester bond hydrolysis

Cellular Component

GO:0005634	nucleus
GO:0005829	cytosol

Protein Structure and Domains

PDB ID

MGI:1919353

InterPro

IPR008984 SMAD/FHA domain
IPR019406 Zinc finger, C2H2, APLF-like

PFAM

PF10283

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q9D842

PhosphoSite

PhosphoSite-Q9D842

TrEMBL

UniProt Splice Variant

Entrez Gene

72103

UniGene

RefSeq

NP_077213

MGI ID

MGI Symbol

Aplf

OMIM

CCDS

CCDS20324

HPRD

IMGT

EMBL

AK008513 AK034191 BC002179

GenPept

AAH02179 BAB25711 BAC28625