InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Lats2

Summary

InnateDB Protein

IDBP-168885.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Lats2

Protein Name

large tumor suppressor 2

Synonyms

4932411G09Rik; AV277261; AW228608;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000022531

InnateDB Gene

IDBG-168883 (Lats2)

Protein Structure

UniProt Annotation

Function

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ (By similarity). {ECO:0000250}.

Subcellular Localization

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269PubMed:15343267}. Cytoplasm {ECO:0000269PubMed:15343267}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000269PubMed:15343267}. Nucleus {ECO:0000250}. Note=Colocalizes with AURKA at the centrosomes during interphase, early prophase and cytokinesis (By similarity). Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. Translocates to the nucleus upon mitotic stress by nocodazole treatment (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Expressed at high levels in ovary and testis and at lower levels in all other tissues examined. {ECO:0000269PubMed:10673337}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 7 experimentally validated interaction(s) in this database.
They are also associated with 25 interaction(s) predicted by orthology.

Experimentally validated
Total	7 [view]
Protein-Protein	7 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	25 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0046872	metal ion binding

Biological Process

GO:0000082	G1/S transition of mitotic cell cycle
GO:0006468	protein phosphorylation
GO:0007067	mitotic nuclear division
GO:0009755	hormone-mediated signaling pathway
GO:0030216	keratinocyte differentiation
GO:0034613	cellular protein localization
GO:0035329	hippo signaling
GO:0035556	intracellular signal transduction
GO:0045736	negative regulation of cyclin-dependent protein serine/threonine kinase activity
GO:0046620	regulation of organ growth
GO:0090090	negative regulation of canonical Wnt signaling pathway

Cellular Component

GO:0000922	spindle pole
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005815	microtubule organizing center

Protein Structure and Domains

PDB ID

MGI:1354386

InterPro

IPR000719 Protein kinase domain
IPR000961 AGC-kinase, C-terminal
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain
IPR009060 UBA-like
IPR011009 Protein kinase-like domain
IPR015940 Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote
IPR017892 Protein kinase, C-terminal
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM

PF00069
PF07714
PF00433

PRINTS

PR00109

PIRSF

SMART

SM00133
SM00220
SM00165
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt