InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Pam

Summary

InnateDB Protein

IDBP-184780.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Pam

Protein Name

peptidylglycine alpha-amidating monooxygenase

Synonyms

PHM;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000057112

InnateDB Gene

IDBG-184778 (Pam)

Protein Structure

UniProt Annotation

Function

Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity (By similarity). {ECO:0000250}.

Subcellular Localization

Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}. Note=Secretory granules.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 8 interaction(s) predicted by orthology.

Predicted by orthology
Total	8 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003824	catalytic activity
GO:0004497	monooxygenase activity
GO:0004504	peptidylglycine monooxygenase activity
GO:0004598	peptidylamidoglycolate lyase activity
GO:0005507	copper ion binding
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016715	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen
GO:0031418	L-ascorbic acid binding

Biological Process

GO:0001519	peptide amidation
GO:0006518	peptide metabolic process
GO:0055114	oxidation-reduction process

Cellular Component

GO:0005829	cytosol
GO:0005887	integral component of plasma membrane
GO:0016020	membrane
GO:0030658	transport vesicle membrane
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

MGI:97475

InterPro

IPR000323 Copper type II, ascorbate-dependent monooxygenase, N-terminal
IPR000720 Peptidyl-glycine alpha-amidating monooxygenase
IPR001258 NHL repeat
IPR008977 PHM/PNGase F domain
IPR013017 NHL repeat, subgroup

PFAM

PF01082
PF01436

PRINTS

PR00790

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P97467