InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Usp44

Summary

InnateDB Protein

IDBP-186301.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Usp44

Protein Name

ubiquitin specific peptidase 44

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000092975

InnateDB Gene

IDBG-186299 (Usp44)

Protein Structure

UniProt Annotation

Function

Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination. {ECO:0000269PubMed:21853124}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:21853124}. Note=Peaks in interphase, with relatively low levels maintained throughout mitosis.

Disease Associations

Tissue Specificity

Widely expressed. Highly expressed in lung, pancreas, skin, liver, stomach and intestine. {ECO:0000269PubMed:20402667}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.

Experimentally validated
Total	1 [view]
Protein-Protein	1 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004221	ubiquitin thiolesterase activity
GO:0004843	ubiquitin-specific protease activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0036459	ubiquitinyl hydrolase activity

Biological Process

GO:0006511	ubiquitin-dependent protein catabolic process
GO:0016579	protein deubiquitination
GO:0051322	anaphase
GO:0060564	negative regulation of mitotic anaphase-promoting complex activity
GO:0090231	regulation of spindle checkpoint

Cellular Component

GO:0005634

nucleus

Protein Structure and Domains

PDB ID

MGI:3045318

InterPro

IPR001394 Peptidase C19, ubiquitin carboxyl-terminal hydrolase
IPR001607 Zinc finger, UBP-type
IPR011011 Zinc finger, FYVE/PHD-type

PFAM

PF00443
PF02148

PRINTS

PIRSF

SMART

SM00290

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

PhosphoSite

PhosphoSite-Q8C2S0

TrEMBL

UniProt Splice Variant

Entrez Gene

327799

UniGene

Mm.276331

RefSeq

NP_899022

MGI ID

MGI Symbol

Usp44

OMIM

CCDS

CCDS24129

HPRD

IMGT

EMBL

GenPept