InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Vps4a

Summary

InnateDB Protein

IDBP-190372.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Vps4a

Protein Name

vacuolar protein sorting 4a (yeast)

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000034388

InnateDB Gene

IDBG-190370 (Vps4a)

Protein Structure

UniProt Annotation

Function

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (By similarity). {ECO:0000250}.

Subcellular Localization

Prevacuolar compartment membrane {ECO:0000269PubMed:12594041}; Peripheral membrane protein {ECO:0000269PubMed:12594041}. Late endosome membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Midbody {ECO:0000250}. Note=Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells. Localizes to the midbody of dividing cells, interaction with ZFYVE19/ANCHR mediates retention at midbody. Localized in two distinct rings on either side of the Fleming body (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Highly expressed in testis and moderately in heart and brain. Not detected in spleen, lung, liver, skeletal muscle or kidney. {ECO:0000269PubMed:12594041}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 5 experimentally validated interaction(s) in this database.
They are also associated with 17 interaction(s) predicted by orthology.

Experimentally validated
Total	5 [view]
Protein-Protein	5 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	17 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0009378	four-way junction helicase activity
GO:0016887	ATPase activity
GO:0017111	nucleoside-triphosphatase activity

Biological Process

GO:0006281	DNA repair
GO:0006310	DNA recombination
GO:0008152	metabolic process
GO:0009838	abscission
GO:0015031	protein transport
GO:0016192	vesicle-mediated transport
GO:0031565	cytokinesis checkpoint
GO:0032466	negative regulation of cytokinesis
GO:0051301	cell division

Cellular Component

GO:0005737	cytoplasm
GO:0030496	midbody
GO:0031902	late endosome membrane
GO:0048471	perinuclear region of cytoplasm

Protein Structure and Domains

PDB ID

MGI:1890520

InterPro

IPR002611 IstB-like ATP-binding protein
IPR003593 AAA+ ATPase domain
IPR003959 ATPase, AAA-type, core
IPR007330 MIT
IPR008824 DNA helicase, Holliday junction RuvB type, N-terminal
IPR011704 ATPase, dynein-related, AAA domain
IPR015415 Vps4 oligomerisation, C-terminal
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM