InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Vprbp

Summary

InnateDB Protein

IDBP-196671.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Vprbp

Protein Name

Vpr (HIV-1) binding protein

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000060025

InnateDB Gene

IDBG-196669 (Vprbp)

Protein Structure

UniProt Annotation

Function

Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification. Probable substrate-specific adapter of a DCX (DDB1- CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1- DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2. Involved in the turnover of methylated proteins: recognizes and binds methylated proteins via its chromo domain, leading to ubiquitination of target proteins by the RBX1-DDB1-DCAF1/VPRBP complex. Also part of the EDVP complex, an E3 ligase complex that mediates ubiquitination of proteins such as TERT, leading to TERT degradation and telomerase inhibition. Also acts as an atypical serine/threonine-protein kinase that specifically mediates phosphorylation of 'Thr-120' of histone H2A (H2AT120ph) in a nucleosomal context, thereby repressing transcription. H2AT120ph is present in the regulatory region of many tumor suppresor genes, down-regulates their transcription and is present at high level in a number of tumors. Involved in JNK-mediated apoptosis during cell competition process via its interaction with LLGL1 and LLGL2 (By similarity). The CUL4A-RBX1-DDB1-DCAF1/VPRBP complex is also involved in B-cell development: VPRBP is recruited by RAG1 to ubiquitinate proteins, leading to limit error-prone repair during V(D)J recombination. {ECO:0000250, ECO:0000269PubMed:22157821}.

Subcellular Localization

Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. Note=Associated with chromatin in a DDB1- independent and cell cycle-dependent manner: recruited to chromatin as DNA is being replicated and is released from chromatin before mitosis. {ECO:0000250}.

Disease Associations

Tissue Specificity

Ubiquitously expressed. {ECO:0000269PubMed:11223251}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 44 interaction(s) predicted by orthology.

Experimentally validated
Total	1 [view]
Protein-Protein	1 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	44 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005488	binding
GO:0005515	protein binding
GO:0005524	ATP binding
GO:1990244	histone kinase activity (H2A-T120 specific)

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0006351	transcription, DNA-templated
GO:0016032	viral process
GO:0016567	protein ubiquitination
GO:0030183	B cell differentiation
GO:0033151	V(D)J recombination
GO:0035212	cell competition in a multicellular organism
GO:1990245	histone H2A-T120 phosphorylation

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm

Protein Structure and Domains

PDB ID

MGI:2445220

InterPro

IPR006594 LisH dimerisation motif
IPR016024 Armadillo-type fold
IPR017986 WD40-repeat-containing domain

PFAM

PRINTS

PIRSF

SMART

SM00667

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q80TR8

PhosphoSite

PhosphoSite-Q80TR8

TrEMBL

UniProt Splice Variant

Entrez Gene

321006

UniGene

Mm.489783

RefSeq

MGI ID

MGI Symbol

Vprbp

OMIM

CCDS

HPRD

IMGT

EMBL

AK029372 AK122372 AK135721 BC065119

GenPept

AAH65119 BAC26425 BAC65654 BAE22628