InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Spast

Summary

InnateDB Protein

IDBP-197795.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Spast

Protein Name

spastin

Synonyms

mKIAA1083; Spg4;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000024869

InnateDB Gene

IDBG-197793 (Spast)

Protein Structure

UniProt Annotation

Function

ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis (By similarity). Required for development of axonal processes and for axonal branching. {ECO:0000250, ECO:0000269PubMed:18234839, ECO:0000269PubMed:19141076}.

Subcellular Localization

Membrane {ECO:0000255HAMAP-Rule:MF_03021}; Single-pass membrane protein {ECO:0000255HAMAP-Rule:MF_03021}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000255HAMAP-Rule:MF_03021}. Cytoplasm, cytoskeleton {ECO:0000255HAMAP-Rule:MF_03021}. Cytoplasm, perinuclear region {ECO:0000255HAMAP-Rule:MF_03021}. Endoplasmic reticulum {ECO:0000255HAMAP-Rule:MF_03021}. Endosome {ECO:0000255HAMAP- Rule:MF_03021}. Nucleus {ECO:0000255HAMAP-Rule:MF_03021}. Cytoplasm, cytoskeleton, spindle {ECO:0000255HAMAP- Rule:MF_03021}. Note=Localization to the centrosome is independent of microtubules. Localizes to the midbody of dividing cells, and this requires CHMP1B (By similarity). Evenly distributed along early axons and concentrates in the growth cone of the axons of late stage 3 neurons. {ECO:0000250, ECO:0000269PubMed:18234839}.

Disease Associations

Tissue Specificity

Expressed in brain, heart, liver, lung, skeletal muscle, spinal cord, spleen and testis. {ECO:0000269PubMed:10610178, ECO:0000269PubMed:16026783}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 6 interaction(s) predicted by orthology.

Predicted by orthology
Total	6 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008017	microtubule binding
GO:0008568	microtubule-severing ATPase activity
GO:0009378	four-way junction helicase activity
GO:0017111	nucleoside-triphosphatase activity
GO:0043014	alpha-tubulin binding
GO:0048487	beta-tubulin binding

Biological Process

GO:0001578	microtubule bundle formation
GO:0006200	ATP catabolic process
GO:0006281	DNA repair
GO:0006310	DNA recombination
GO:0006888	ER to Golgi vesicle-mediated transport
GO:0007109	cytokinesis, completion of separation
GO:0007409	axonogenesis
GO:0031117	positive regulation of microtubule depolymerization
GO:0034214	protein hexamerization
GO:0051013	microtubule severing
GO:0051260	protein homooligomerization

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005768	endosome
GO:0005783	endoplasmic reticulum
GO:0005813	centrosome
GO:0005819	spindle
GO:0005874	microtubule
GO:0015630	microtubule cytoskeleton
GO:0016021	integral component of membrane
GO:0030496	midbody
GO:0031410	cytoplasmic vesicle
GO:0048471	perinuclear region of cytoplasm
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

MGI:1858896

InterPro

IPR003593 AAA+ ATPase domain
IPR003959 ATPase, AAA-type, core
IPR007330 MIT
IPR008824 DNA helicase, Holliday junction RuvB type, N-terminal
IPR017179 Spastin
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00004
PF07724
PF13304
PF04212
PF05496

PRINTS

PIRSF

PIRSF037338

SMART

SM00382
SM00745

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt