InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Rpa1

Summary

InnateDB Protein

IDBP-199729.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Rpa1

Protein Name

replication protein A1

Synonyms

5031405K23Rik; 70kDa; AA589576; AW557552; RF-A; RP-A; Rpa;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000090585

InnateDB Gene

IDBG-199725 (Rpa1)

Protein Structure

UniProt Annotation

Function

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus {ECO:0000250}. Nucleus, PML body {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 12 experimentally validated interaction(s) in this database.
They are also associated with 104 interaction(s) predicted by orthology.

Experimentally validated
Total	12 [view]
Protein-Protein	12 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	104 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003682	chromatin binding
GO:0003697	single-stranded DNA binding
GO:0046872	metal ion binding

Biological Process

GO:0000724	double-strand break repair via homologous recombination
GO:0001701	in utero embryonic development
GO:0006260	DNA replication
GO:0006284	base-excision repair
GO:0006289	nucleotide-excision repair
GO:0006298	mismatch repair
GO:0007126	meiotic nuclear division
GO:0008284	positive regulation of cell proliferation
GO:0030097	hemopoiesis
GO:0048873	homeostasis of number of cells within a tissue
GO:0051276	chromosome organization

Cellular Component

GO:0000793	condensed chromosome
GO:0000794	condensed nuclear chromosome
GO:0000800	lateral element
GO:0001673	male germ cell nucleus
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005662	DNA replication factor A complex

Protein Structure and Domains

PDB ID

MGI:1915525

InterPro

IPR004365 OB-fold nucleic acid binding domain, AA-tRNA synthetase-type
IPR004591 Replication factor-a protein 1 Rpa1
IPR007199 Replication factor-A protein 1, N-terminal
IPR012340 Nucleic acid-binding, OB-fold
IPR013955 Replication factor A, C-terminal

PFAM

PF01336
PF04057
PF08646

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q8VEE4

PhosphoSite