InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Prpf8

Summary

InnateDB Protein

IDBP-200021.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Prpf8

Protein Name

pre-mRNA processing factor 8

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000099568

InnateDB Gene

IDBG-200019 (Prpf8)

Protein Structure

UniProt Annotation

Function

Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus speckle {ECO:0000269PubMed:15169873}.

Disease Associations

Tissue Specificity

Strongly expressed in testis (preferentially in the outer cell layer), and moderately in ovary (preferentially in granulosa cells). {ECO:0000269PubMed:11275560}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 61 experimentally validated interaction(s) in this database.
They are also associated with 89 interaction(s) predicted by orthology.

Experimentally validated
Total	61 [view]
Protein-Protein	61 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	89 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003723	RNA binding
GO:0005515	protein binding
GO:0017070	U6 snRNA binding
GO:0030623	U5 snRNA binding
GO:0046982	protein heterodimerization activity

Biological Process

GO:0000398

mRNA splicing, via spliceosome

Cellular Component

GO:0016607	nuclear speck
GO:0071013	catalytic step 2 spliceosome

Protein Structure and Domains

PDB ID

MGI:2179381

InterPro

IPR000555 JAB1/MPN/MOV34 metalloenzyme domain
IPR008972 Cupredoxin
IPR009072 Histone-fold
IPR012591 PRO8NT domain
IPR012592 PROCN domain
IPR012984 PROCT domain
IPR019580 Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding
IPR019581 Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding
IPR019582 RNA recognition motif, spliceosomal PrP8
IPR021983 PRP8 domain IV core

PFAM