InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Tex14

Summary

InnateDB Protein

IDBP-207321.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Tex14

Protein Name

testis expressed gene 14

Synonyms

C85585;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000054444

InnateDB Gene

IDBG-207319 (Tex14)

Protein Structure

UniProt Annotation

Function

Required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges. Also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Has no protein kinase activity in vitro. {ECO:0000269PubMed:16549803, ECO:0000269PubMed:19020301, ECO:0000269PubMed:20176808, ECO:0000269PubMed:22405274}.

Subcellular Localization

Cytoplasm. Midbody. Chromosome, centromere, kinetochore. Note=Detected in the intercellular bridges that connect male germ cell daughter cells after cell division.

Disease Associations

Tissue Specificity

Detected in testis and spermatogonia. Not detectable in the other tissues tested. {ECO:0000269PubMed:11279525, ECO:0000269PubMed:12711554, ECO:0000269PubMed:16549803}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 5 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	5 [view]
Protein-Protein	5 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0019901	protein kinase binding

Biological Process

GO:0006468	protein phosphorylation
GO:0007094	mitotic spindle assembly checkpoint
GO:0007140	male meiosis
GO:0008608	attachment of spindle microtubules to kinetochore
GO:0032091	negative regulation of protein binding
GO:0032466	negative regulation of cytokinesis
GO:0043063	intercellular bridge organization
GO:0051306	mitotic sister chromatid separation

Cellular Component

GO:0000776	kinetochore
GO:0000777	condensed chromosome kinetochore
GO:0005623	cell
GO:0005737	cytoplasm
GO:0030496	midbody
GO:0045171	intercellular bridge

Protein Structure and Domains

PDB ID

MGI:1933227

InterPro

IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002110 Ankyrin repeat
IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR020635 Tyrosine-protein kinase, catalytic domain
IPR020683 Ankyrin repeat-containing domain

PFAM

PF00069
PF07714
PF00023
PF13606
PF11929
PF12796

PRINTS

PR00109
PR01415

PIRSF

SMART

SM00248
SM00220
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt