InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Rac1

Summary

InnateDB Protein

IDBP-207765.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Rac1

Protein Name

RAS-related C3 botulinum substrate 1

Synonyms

AL023026; D5Ertd559e;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000079380

InnateDB Gene

IDBG-207763 (Rac1)

Protein Structure

UniProt Annotation

Function

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3. {ECO:0000269PubMed:19029984}.

Subcellular Localization

Cell membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Melanosome {ECO:0000250}. Cytoplasm {ECO:0000250}. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone- resorbing osteoclasts (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Widely expressed. {ECO:0000269PubMed:1905006}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 58 experimentally validated interaction(s) in this database.
They are also associated with 148 interaction(s) predicted by orthology.

Experimentally validated
Total	58 [view]
Protein-Protein	56 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	2 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	148 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003924	GTPase activity
GO:0005515	protein binding
GO:0005525	GTP binding
GO:0017137	Rab GTPase binding
GO:0019899	enzyme binding
GO:0019901	protein kinase binding
GO:0030742	GTP-dependent protein binding
GO:0031996	thioesterase binding
GO:0051022	Rho GDP-dissociation inhibitor binding

Biological Process

GO:0001934	positive regulation of protein phosphorylation
GO:0002093	auditory receptor cell morphogenesis
GO:0002551	mast cell chemotaxis
GO:0003382	epithelial cell morphogenesis
GO:0006184	GTP catabolic process
GO:0006897	endocytosis
GO:0006911	phagocytosis, engulfment
GO:0006928	cellular component movement
GO:0006935	chemotaxis
GO:0006972	hyperosmotic response
GO:0007010	cytoskeleton organization
GO:0007015	actin filament organization
GO:0007155	cell adhesion
GO:0007165	signal transduction
GO:0007186	G-protein coupled receptor signaling pathway
GO:0007264	small GTPase mediated signal transduction
GO:0007411	axon guidance
GO:0008283	cell proliferation
GO:0010592	positive regulation of lamellipodium assembly
GO:0015031	protein transport
GO:0016358	dendrite development
GO:0016477	cell migration
GO:0021799	cerebral cortex radially oriented cell migration
GO:0021831	embryonic olfactory bulb interneuron precursor migration
GO:0030032	lamellipodium assembly
GO:0030036	actin cytoskeleton organization
GO:0030041	actin filament polymerization
GO:0030334	regulation of cell migration
GO:0030838	positive regulation of actin filament polymerization
GO:0031529	ruffle organization
GO:0032707	negative regulation of interleukin-23 production
GO:0034446	substrate adhesion-dependent cell spreading
GO:0043552	positive regulation of phosphatidylinositol 3-kinase activity
GO:0043652	engulfment of apoptotic cell
GO:0045087	innate immune response (InnateDB)
GO:0045216	cell-cell junction organization
GO:0045453	bone resorption
GO:0045740	positive regulation of DNA replication
GO:0048532	anatomical structure arrangement
GO:0048813	dendrite morphogenesis
GO:0048870	cell motility
GO:0051668	localization within membrane
GO:0060071	Wnt signaling pathway, planar cell polarity pathway
GO:0060263	regulation of respiratory burst
GO:0071526	semaphorin-plexin signaling pathway
GO:0071542	dopaminergic neuron differentiation
GO:0072659	protein localization to plasma membrane
GO:0090103	cochlea morphogenesis
GO:0097178	ruffle assembly

Cellular Component

GO:0000139	Golgi membrane
GO:0001891	phagocytic cup
GO:0005622	intracellular
GO:0005737	cytoplasm
GO:0005802	trans-Golgi network
GO:0005829	cytosol
GO:0016020	membrane
GO:0016023	cytoplasmic membrane-bounded vesicle
GO:0019897	extrinsic component of plasma membrane
GO:0030027	lamellipodium
GO:0031410	cytoplasmic vesicle
GO:0032587	ruffle membrane
GO:0036464	cytoplasmic ribonucleoprotein granule
GO:0042470	melanosome
GO:0042995	cell projection
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

MGI:97845

InterPro

IPR001806 Small GTPase superfamily
IPR003578 Small GTPase superfamily, Rho type
IPR003579 Small GTPase superfamily, Rab type
IPR005225 Small GTP-binding protein domain
IPR006689 Small GTPase superfamily, ARF/SAR type
IPR013684 Mitochondrial Rho-like
IPR020849 Small GTPase superfamily, Ras type
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00071
PF00025
PF08477

PRINTS

PR00449
PR00328

PIRSF

SMART

SM00174
SM00175
SM00173

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt