InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Alyref

Summary

InnateDB Protein

IDBP-214847.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Alyref

Protein Name

THO complex 4

Synonyms

ALY; REF1; Refbp1; Thoc4;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000026125

InnateDB Gene

IDBG-214845 (Alyref)

Protein Structure

UniProt Annotation

Function

Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability.Acts as chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation.

Subcellular Localization

Nucleus {ECO:0000269PubMed:9119228}. Nucleus speckle {ECO:0000250}. Cytoplasm {ECO:0000269PubMed:9119228}. Note=Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and DDX39B in the nucleus and nuclear speckles. Localizes to regions surrounding nuclear speckles known as perispeckles in which TREX complex assembly seems to occur (By similarity). Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. {ECO:0000250}.

Disease Associations

Tissue Specificity

Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. {ECO:0000269PubMed:9119228}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 11 experimentally validated interaction(s) in this database.
They are also associated with 70 interaction(s) predicted by orthology.