InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: NCF1

Summary

InnateDB Protein

IDBP-21572.7

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

NCF1

Protein Name

neutrophil cytosolic factor 1

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000289473

InnateDB Gene

IDBG-21570 (NCF1)

Protein Structure

UniProt Annotation

Function

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). {ECO:0000269PubMed:19801500, ECO:0000269PubMed:2547247, ECO:0000269PubMed:2550933}.

Subcellular Localization

Cytoplasm, cytosol. Membrane; Peripheral membrane protein; Cytoplasmic side.

Disease Associations

Granulomatous disease, chronic, cytochrome-b-positive 1, autosomal recessive (CGD1) [MIM:233700]: A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. {ECO:0000269PubMed:11133775}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Tissue Specificity

Detected in peripheral blood monocytes and neutrophils (at protein level). {ECO:0000269PubMed:2547247, ECO:0000269PubMed:2550933}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 68 experimentally validated interaction(s) in this database.
They are also associated with 3 interaction(s) predicted by orthology.

Experimentally validated
Total	68 [view]
Protein-Protein	67 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	3 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0009055	electron carrier activity
GO:0016175	superoxide-generating NADPH oxidase activity
GO:0017124	SH3 domain binding
GO:0035091	phosphatidylinositol binding
GO:0043325	phosphatidylinositol-3,4-bisphosphate binding

Biological Process

GO:0001878	response to yeast
GO:0001909	leukocyte mediated cytotoxicity
GO:0002474	antigen processing and presentation of peptide antigen via MHC class I
GO:0002479	antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
GO:0002679	respiratory burst involved in defense response
GO:0006612	protein targeting to membrane
GO:0006691	leukotriene metabolic process
GO:0006801	superoxide metabolic process
GO:0006915	apoptotic process
GO:0006954	inflammatory response
GO:0006968	cellular defense response
GO:0008283	cell proliferation
GO:0009617	response to bacterium
GO:0042554	superoxide anion generation
GO:0042590	antigen processing and presentation of exogenous peptide antigen via MHC class I
GO:0042742	defense response to bacterium
GO:0045087	innate immune response
GO:0045730	respiratory burst
GO:0045986	negative regulation of smooth muscle contraction
GO:0050665	hydrogen peroxide biosynthetic process
GO:0050830	defense response to Gram-positive bacterium
GO:0050832	defense response to fungus
GO:0051701	interaction with host
GO:0055114	oxidation-reduction process
GO:0070946	neutrophil mediated killing of gram-positive bacterium
GO:0070947	neutrophil mediated killing of fungus
GO:0090382	phagosome maturation

Cellular Component

GO:0005737	cytoplasm
GO:0005791	rough endoplasmic reticulum
GO:0005794	Golgi apparatus
GO:0005829	cytosol
GO:0005886	plasma membrane
GO:0016020	membrane
GO:0019898	extrinsic component of membrane
GO:0030425	dendrite
GO:0032010	phagolysosome
GO:0043020	NADPH oxidase complex
GO:0043025	neuronal cell body

Protein Structure and Domains

PDB ID

InterPro

IPR001452 SH3 domain
IPR001655 Neutrophil cytosol factor 1
IPR001683 Phox homologous domain
IPR011511 Variant SH3 domain
IPR015039 NADPH oxidase subunit p47Phox, C-terminal

PFAM

PF00018
PF14604
PF00787
PF07653
PF08944

PRINTS

PR00452
PR00498

PIRSF

SMART

SM00326
SM00312

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt