Version 5.4
| Homo sapiens Protein: TOP3B | |||||||||||||||||||
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| Summary | |||||||||||||||||||
| InnateDB Protein | IDBP-2168.6 | ||||||||||||||||||
| Last Modified | 2014-10-13 [Report errors or provide feedback] | ||||||||||||||||||
| Gene Symbol | TOP3B | ||||||||||||||||||
| Protein Name | topoisomerase (DNA) III beta | ||||||||||||||||||
| Synonyms | TOP3B1; | ||||||||||||||||||
| Species | Homo sapiens | ||||||||||||||||||
| Ensembl Protein | ENSP00000349705 | ||||||||||||||||||
| InnateDB Gene | IDBG-2166 (TOP3B) | ||||||||||||||||||
| Protein Structure |
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| UniProt Annotation | |||||||||||||||||||
| Function | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity. {ECO:0000250}. | ||||||||||||||||||
| Subcellular Localization | |||||||||||||||||||
| Disease Associations | |||||||||||||||||||
| Tissue Specificity | Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas. {ECO:0000269PubMed:9927731}. | ||||||||||||||||||
| Comments | |||||||||||||||||||
| Interactions | |||||||||||||||||||
| Number of Interactions |
This gene and/or its encoded proteins are associated with 17 experimentally validated interaction(s) in this database.
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| Gene Ontology | |||||||||||||||||||
Molecular Function |
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| Biological Process |
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| Cellular Component |
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| Protein Structure and Domains | |||||||||||||||||||
| PDB ID | |||||||||||||||||||
| InterPro |
IPR000380
DNA topoisomerase, type IA IPR003601 DNA topoisomerase, type IA, domain 2 IPR003602 DNA topoisomerase, type IA, DNA-binding domain IPR006171 Toprim domain IPR013497 DNA topoisomerase, type IA, central IPR023405 DNA topoisomerase, type IA, core domain |
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| PFAM |
PF01751
PF13662 PF01131 |
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| PRINTS |
PR00417
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| PIRSF | |||||||||||||||||||
| SMART |
SM00436
SM00437 SM00493 |
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| TIGRFAMs | |||||||||||||||||||
| Post-translational Modifications | |||||||||||||||||||
| Modification | |||||||||||||||||||
| Cross-References | |||||||||||||||||||
| SwissProt | O95985 | ||||||||||||||||||
| PhosphoSite | PhosphoSite-O95985 | ||||||||||||||||||
| TrEMBL | C9JTQ7 | ||||||||||||||||||
| UniProt Splice Variant | |||||||||||||||||||
| Entrez Gene | 8940 | ||||||||||||||||||
| UniGene | Hs.436401 | ||||||||||||||||||
| RefSeq | NP_001269042 | ||||||||||||||||||
| HUGO | HGNC:11993 | ||||||||||||||||||
| OMIM | 603582 | ||||||||||||||||||
| CCDS | CCDS13797 | ||||||||||||||||||
| HPRD | 04662 | ||||||||||||||||||
| IMGT | |||||||||||||||||||
| EMBL | AF017146 AF053082 AF125216 BC002432 CH471095 CR456596 D87012 | ||||||||||||||||||
| GenPept | AAD01614 AAD15791 AAD29670 AAH02432 BAA20009 CAG30482 EAW59487 EAW59488 EAW59490 EAW59491 | ||||||||||||||||||