InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: ALOX12

Summary

InnateDB Protein

IDBP-22833.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

ALOX12

Protein Name

arachidonate 12-lipoxygenase

Synonyms

12-LOX; 12S-LOX; LOG12;

Species

Homo sapiens

Ensembl Protein

ENSP00000251535

InnateDB Gene

IDBG-22829 (ALOX12)

Protein Structure

UniProt Annotation

Function

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation. {ECO:0000269PubMed:16638750, ECO:0000269PubMed:22237009, ECO:0000269PubMed:23578768, ECO:0000269PubMed:8250832, ECO:0000269PubMed:9751607}.

Subcellular Localization

Cytoplasm, cytosol. Membrane. Note=Membrane association is stimulated by EGF.

Disease Associations

Esophageal cancer (ESCR) [MIM:133239]: A malignancy of the esophagus. The most common types are esophageal squamous cell carcinoma and adenocarcinoma. Cancer of the esophagus remains a devastating disease because it is usually not detected until it has progressed to an advanced incurable stage. {ECO:0000269PubMed:17460548}. Note=Disease susceptibility may be associated with variations affecting the gene represented in this entry. Gln at position 261 may confer interindividual susceptibility to esophageal cancer (PubMed:17460548). {ECO:0000269PubMed:17460548}.Colorectal cancer (CRC) [MIM:114500]: A complex disease characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum. Genetic alterations are often associated with progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history. {ECO:0000269PubMed:17151091}. Note=Disease susceptibility may be associated with variations affecting the gene represented in this entry. Gln at position 261 may confer interindividual susceptibility to colorectal cancer (PubMed:17460548). {ECO:0000269PubMed:17460548}.

Tissue Specificity

Expressed in vascular smooth muscle cells. {ECO:0000269PubMed:23578768}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 13 experimentally validated interaction(s) in this database.

Experimentally validated
Total	13 [view]
Protein-Protein	11 [view]
Protein-DNA	2 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004052	arachidonate 12-lipoxygenase activity
GO:0005506	iron ion binding
GO:0005515	protein binding
GO:0016165	lipoxygenase activity
GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0046872	metal ion binding
GO:0047977	hepoxilin-epoxide hydrolase activity
GO:0051120	hepoxilin A3 synthase activity

Biological Process

GO:0006928	cellular component movement
GO:0007568	aging
GO:0008284	positive regulation of cell proliferation
GO:0010595	positive regulation of endothelial cell migration
GO:0010628	positive regulation of gene expression
GO:0010656	negative regulation of muscle cell apoptotic process
GO:0010942	positive regulation of cell death
GO:0019369	arachidonic acid metabolic process
GO:0019372	lipoxygenase pathway
GO:0019395	fatty acid oxidation
GO:0030307	positive regulation of cell growth
GO:0030335	positive regulation of cell migration
GO:0042554	superoxide anion generation
GO:0043065	positive regulation of apoptotic process
GO:0043066	negative regulation of apoptotic process
GO:0043280	positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0043651	linoleic acid metabolic process
GO:0044281	small molecule metabolic process
GO:0045603	positive regulation of endothelial cell differentiation
GO:0045766	positive regulation of angiogenesis
GO:0045785	positive regulation of cell adhesion
GO:0045909	positive regulation of vasodilation
GO:0048661	positive regulation of smooth muscle cell proliferation
GO:0051121	hepoxilin metabolic process
GO:0051122	hepoxilin biosynthetic process
GO:0051901	positive regulation of mitochondrial depolarization
GO:0055114	oxidation-reduction process
GO:0061436	establishment of skin barrier
GO:0071396	cellular response to lipid
GO:0072593	reactive oxygen species metabolic process
GO:0090331	negative regulation of platelet aggregation
GO:1901751	leukotriene A4 metabolic process
GO:2001300	lipoxin metabolic process
GO:2001303	lipoxin A4 biosynthetic process
GO:2001306	lipoxin B4 biosynthetic process