Homo sapiens Protein: GNAT3 | |||||||||||||||||||||||||||||||
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Summary | |||||||||||||||||||||||||||||||
InnateDB Protein | IDBP-233447.5 | ||||||||||||||||||||||||||||||
Last Modified | 2014-10-13 [Report errors or provide feedback] | ||||||||||||||||||||||||||||||
Gene Symbol | GNAT3 | ||||||||||||||||||||||||||||||
Protein Name | guanine nucleotide binding protein, alpha transducing 3 | ||||||||||||||||||||||||||||||
Synonyms | |||||||||||||||||||||||||||||||
Species | Homo sapiens | ||||||||||||||||||||||||||||||
Ensembl Protein | ENSP00000381339 | ||||||||||||||||||||||||||||||
InnateDB Gene | IDBG-233445 (GNAT3) | ||||||||||||||||||||||||||||||
Protein Structure | |||||||||||||||||||||||||||||||
UniProt Annotation | |||||||||||||||||||||||||||||||
Function | Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide- 1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity. {ECO:0000269PubMed:11917125, ECO:0000269PubMed:17724330}. | ||||||||||||||||||||||||||||||
Subcellular Localization | Cytoplasm {ECO:0000269PubMed:8015379}. Note=Dual ditribution pattern; plasmalemmal pattern with apical region localization and cytosolic pattern with localization throughout the cytoplasm. | ||||||||||||||||||||||||||||||
Disease Associations | |||||||||||||||||||||||||||||||
Tissue Specificity | Expressed in taste buds (sensory organs of clustered epithelial cells) of the circumvallate and foliate papillae of the tongue at protein level. Expressed in enteroendocrine L cells of the gut. Detected also in spermatozoa. {ECO:0000269PubMed:16728727, ECO:0000269PubMed:17021831, ECO:0000269PubMed:17724332, ECO:0000269PubMed:8015379}. | ||||||||||||||||||||||||||||||
Comments | |||||||||||||||||||||||||||||||
Interactions | |||||||||||||||||||||||||||||||
Number of Interactions |
This gene and/or its encoded proteins are associated with 4 experimentally validated interaction(s) in this database.
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Gene Ontology | |||||||||||||||||||||||||||||||
Molecular Function |
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Biological Process |
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Cellular Component |
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Protein Structure and Domains | |||||||||||||||||||||||||||||||
PDB ID | |||||||||||||||||||||||||||||||
InterPro |
IPR001019
Guanine nucleotide binding protein (G-protein), alpha subunit IPR001408 G-protein alpha subunit, group I IPR006689 Small GTPase superfamily, ARF/SAR type IPR011025 G protein alpha subunit, helical insertion IPR027417 P-loop containing nucleoside triphosphate hydrolase |
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PFAM |
PF00503
PF00025 |
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PRINTS |
PR00318
PR00441 PR00328 |
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PIRSF | |||||||||||||||||||||||||||||||
SMART |
SM00275
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TIGRFAMs | |||||||||||||||||||||||||||||||
Post-translational Modifications | |||||||||||||||||||||||||||||||
Modification | |||||||||||||||||||||||||||||||
Cross-References | |||||||||||||||||||||||||||||||
SwissProt | A8MTJ3 | ||||||||||||||||||||||||||||||
PhosphoSite | PhosphoSite-NP_001095856 | ||||||||||||||||||||||||||||||
TrEMBL | |||||||||||||||||||||||||||||||
UniProt Splice Variant | |||||||||||||||||||||||||||||||
Entrez Gene | 346562 | ||||||||||||||||||||||||||||||
UniGene | Hs.335049 | ||||||||||||||||||||||||||||||
RefSeq | NP_001095856 | ||||||||||||||||||||||||||||||
HUGO | HGNC:22800 | ||||||||||||||||||||||||||||||
OMIM | 139395 | ||||||||||||||||||||||||||||||
CCDS | CCDS47625 | ||||||||||||||||||||||||||||||
HPRD | |||||||||||||||||||||||||||||||
IMGT | |||||||||||||||||||||||||||||||
EMBL | AC004862 BC147016 BC147017 CH236949 CH471091 | ||||||||||||||||||||||||||||||
GenPept | AAI47017 AAI47018 EAL24192 EAL24193 EAW77007 | ||||||||||||||||||||||||||||||