InnateDB Protein
|
IDBP-236984.6
|
Last Modified
|
2014-10-13 [Report errors or provide feedback]
|
Gene Symbol
|
RPA3
|
Protein Name
|
replication protein A3, 14kDa
|
Synonyms
|
REPA3;
|
Species
|
Homo sapiens
|
Ensembl Protein
|
ENSP00000379914
|
InnateDB Gene
|
IDBG-8521 (RPA3)
|
Protein Structure
|
|
Function |
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER), probably through interaction with UNG (PubMed:9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA (PubMed:19010961). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105). {ECO:0000269PubMed:19010961, ECO:0000269PubMed:19116208, ECO:0000269PubMed:19996105, ECO:0000269PubMed:7697716, ECO:0000269PubMed:9430682, ECO:0000269PubMed:9765279}.
|
Subcellular Localization |
Nucleus.
|
Disease Associations |
|
Tissue Specificity |
|
Comments |
|
Number of Interactions
|
This gene and/or its encoded proteins are associated with 388 experimentally validated interaction(s) in this database.
They are also associated with 4 interaction(s) predicted by orthology.
Experimentally validated |
Total |
388
[view]
|
Protein-Protein |
384
[view]
|
Protein-DNA |
2
[view]
|
Protein-RNA |
0
|
DNA-DNA |
2
[view]
|
RNA-RNA |
0
|
DNA-RNA |
0
|
|
Predicted by orthology |
Total |
4 [view]
|
|
|
Molecular Function |
|
Biological Process |
|
Cellular Component |
|
PDB ID |
|
InterPro |
IPR012340
Nucleic acid-binding, OB-fold
IPR013970
Replication factor A protein 3
|
PFAM |
PF08661
|
PRINTS |
|
PIRSF |
|
SMART |
|
TIGRFAMs |
|
Modification |
|
SwissProt |
P35244
|
PhosphoSite |
PhosphoSite-P35244
|
TrEMBL |
B5MC59
|
UniProt Splice Variant |
|
Entrez Gene |
6119
|
UniGene |
Hs.738841
|
RefSeq |
|
HUGO |
HGNC:10291
|
OMIM |
179837
|
CCDS |
CCDS5356
|
HPRD |
01567
|
IMGT |
|
EMBL |
AC004948
AC007161
AK311842
BC005264
BC009868
BT007320
CH236948
CH471073
DQ003136
L07493
|
GenPept |
AAA58350
AAH05264
AAH09868
AAP35984
AAQ96878
AAX84517
BAG34784
EAL24304
EAW93603
EAW93604
EAW93605
|
|
|