InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Rnf128

Summary

InnateDB Protein

IDBP-254744.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Rnf128

Protein Name

ring finger protein 128

Synonyms

1300002C13Rik; AI987883; GRAIL; Greul1;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000108649

InnateDB Gene

IDBG-174238 (Rnf128)

Protein Structure

UniProt Annotation

Function

E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with 'Lys- 48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals (By similarity). {ECO:0000250}.

Subcellular Localization

Endomembrane system {ECO:0000250}; Single- pass membrane protein {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Cytoplasm, perinuclear region {ECO:0000250}. Note=Localized in an asymmetric perinuclear punctate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7A (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Expressed in brain, kidney, heart, liver, ovary, testis and thymus. Expression increased as early as 4 hours by 5- to 7-fold in anergized cultures as compared to resting or activated cells. {ECO:0000269PubMed:12705856}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 4 experimentally validated interaction(s) in this database.
They are also associated with 9 interaction(s) predicted by orthology.

Experimentally validated
Total	4 [view]
Protein-Protein	4 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	9 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity
GO:0046872	metal ion binding

Biological Process

GO:0016567	protein ubiquitination
GO:0042036	negative regulation of cytokine biosynthetic process

Cellular Component

GO:0005770	late endosome
GO:0005783	endoplasmic reticulum
GO:0005794	Golgi apparatus
GO:0005856	cytoskeleton
GO:0016021	integral component of membrane
GO:0048471	perinuclear region of cytoplasm