InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Brap

Summary

InnateDB Protein

IDBP-257429.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Brap

Protein Name

BRCA1 associated protein

Synonyms

3010002G07Rik; BRAP2; IMP;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000107395

InnateDB Gene

IDBG-197596 (Brap)

Protein Structure

UniProt Annotation

Function

Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto- polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport (By similarity). {ECO:0000250UniProtKB:Q7Z569}.

Subcellular Localization

Cytoplasm {ECO:0000250}.

Disease Associations

Tissue Specificity

Isoform 2 is highly expressed in testis, lower levels in brain, heart, lung, stomach, colon, uterus, liver and kidney. Isoform 1 is only expressed in the testis. Isoform 2 is predominant over isoform 1 in both fetal and adult testis. {ECO:0000269PubMed:14607334}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 9 experimentally validated interaction(s) in this database.
They are also associated with 26 interaction(s) predicted by orthology.

Experimentally validated
Total	9 [view]
Protein-Protein	9 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	26 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008139	nuclear localization sequence binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity
GO:0046872	metal ion binding

Biological Process

GO:0000165	MAPK cascade
GO:0007265	Ras protein signal transduction
GO:0009968	negative regulation of signal transduction
GO:0016567	protein ubiquitination

Cellular Component

GO:0000151	ubiquitin ligase complex
GO:0005737	cytoplasm

Protein Structure and Domains

PDB ID

MGI:1919649

InterPro

IPR001607 Zinc finger, UBP-type
IPR001841 Zinc finger, RING-type
IPR011011 Zinc finger, FYVE/PHD-type
IPR011422 BRCA1-associated 2
IPR018957 Zinc finger, C3HC4 RING-type

PFAM

PF02148
PF13639
PF14634
PF07576
PF00097

PRINTS

PIRSF

SMART

SM00290
SM00184

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q99MP8