InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Myo10

Summary

InnateDB Protein

IDBP-260177.7

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Myo10

Protein Name

myosin X

Synonyms

AW048724; D15Ertd600e; mKIAA0799;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000106087

InnateDB Gene

IDBG-130902 (Myo10)

Protein Structure

UniProt Annotation

Function

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. MYO10 binds to actin filaments and actin bundles and functions as plus end-directed motor. The tail domain binds to membranous compartments containing phosphatidylinositol 3,4,5-trisphosphate or integrins, and mediates cargo transport along actin filaments (By similarity). Regulates cell shape, cell spreading and cell adhesion. Stimulates the formation and elongation of filopodia. May play a role in neurite outgrowth and axon guidance. In hippocampal neurons it induces the formation of dendritic filopodia by trafficking the actin-remodeling protein VASP to the tips of filopodia, where it promotes actin elongation. Plays a role in formation of the podosome belt in osteoclasts. {ECO:0000250}.Isoform Headless: Functions as a dominant-negative regulator of isoform 1, suppressing its filopodia-inducing and axon outgrowth-promoting activities. In hippocampal neurons, it increases VASP retention in spine heads to induce spine formation and spine head expansion.

Subcellular Localization

Cytoplasm, cytosol. Cell projection, lamellipodium {ECO:0000250}. Cell projection, ruffle {ECO:0000250}. Cytoplasm, cytoskeleton. Cell projection, filopodium tip. Cytoplasm, cell cortex. Cell projection, filopodium membrane; Peripheral membrane protein. Note=May be in an inactive, monomeric conformation in the cytosol. Detected in cytoplasmic punctae and in cell projections. Colocalizes with actin fibers. Interacts with microtubules. Undergoes forward and rearward movements within filopodia. Interaction with membranes containing phosphatidylinositol 3,4,5-trisphosphate mediates localization at filopodium membranes (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Detected in brain, heart, kidney, liver, stomach, skeletal muscle, lung, testis and skin. Isoform Headless is expressed in embryonic and neuronal stem cells, and enriched in proliferating and migrating cells. {ECO:0000269PubMed:10799329, ECO:0000269PubMed:22661706}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 8 experimentally validated interaction(s) in this database.
They are also associated with 17 interaction(s) predicted by orthology.

Experimentally validated
Total	8 [view]
Protein-Protein	5 [view]
Protein-DNA	2 [view]
Protein-RNA	1 [view]
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	17 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003774	motor activity
GO:0005515	protein binding
GO:0005516	calmodulin binding
GO:0005524	ATP binding
GO:0005547	phosphatidylinositol-3,4,5-trisphosphate binding
GO:0030507	spectrin binding
GO:0030898	actin-dependent ATPase activity
GO:0051015	actin filament binding
GO:0060002	plus-end directed microfilament motor activity

Biological Process

GO:0006200	ATP catabolic process
GO:0007165	signal transduction
GO:0008152	metabolic process
GO:0008360	regulation of cell shape
GO:0022409	positive regulation of cell-cell adhesion
GO:0030705	cytoskeleton-dependent intracellular transport
GO:0048870	cell motility
GO:0051489	regulation of filopodium assembly

Cellular Component

GO:0001726	ruffle
GO:0005730	nucleolus
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005856	cytoskeleton
GO:0005886	plasma membrane
GO:0005938	cell cortex
GO:0016459	myosin complex
GO:0030027	lamellipodium
GO:0030175	filopodium
GO:0031527	filopodium membrane
GO:0032433	filopodium tip
GO:0043005	neuron projection
GO:0043025	neuronal cell body

Protein Structure and Domains

PDB ID

MGI:107716

InterPro

IPR000048 IQ motif, EF-hand binding site
IPR000159 Ras-association
IPR000299 FERM domain
IPR000857 MyTH4 domain
IPR001609 Myosin head, motor domain
IPR001849 Pleckstrin homology domain
IPR019748 FERM central domain
IPR019749 Band 4.1 domain
IPR027417 P-loop containing nucleoside triphosphate hydrolase
IPR029071 Ubiquitin-related domain

PFAM

PF00612
PF00788
PF00784
PF00063
PF00169
PF00373

PRINTS

PR00193

PIRSF

SMART

SM00015
SM00314
SM00139
SM00242
SM00233
SM00295

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt