InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Ruvbl2

Summary

InnateDB Protein

IDBP-265961.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Ruvbl2

Protein Name

RuvB-like protein 2

Synonyms

mp47; p47;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000103400

InnateDB Gene

IDBG-182997 (Ruvbl2)

Protein Structure

UniProt Annotation

Function

Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. {ECO:0000250}.Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome (By similarity). {ECO:0000250}.Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. {ECO:0000250}.Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2 (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 24 experimentally validated interaction(s) in this database.
They are also associated with 195 interaction(s) predicted by orthology.

Experimentally validated
Total	24 [view]
Protein-Protein	24 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	195 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0000979	RNA polymerase II core promoter sequence-specific DNA binding
GO:0000980	RNA polymerase II distal enhancer sequence-specific DNA binding
GO:0003678	DNA helicase activity
GO:0003684	damaged DNA binding
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016887	ATPase activity
GO:0017111	nucleoside-triphosphatase activity
GO:0031490	chromatin DNA binding
GO:0042802	identical protein binding
GO:0043141	ATP-dependent 5'-3' DNA helicase activity

Biological Process

GO:0006200	ATP catabolic process
GO:0006260	DNA replication
GO:0006281	DNA repair
GO:0006310	DNA recombination
GO:0006338	chromatin remodeling
GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0032508	DNA duplex unwinding
GO:0034644	cellular response to UV
GO:0035066	positive regulation of histone acetylation
GO:0043967	histone H4 acetylation
GO:0043968	histone H2A acetylation
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0071169	establishment of protein localization to chromatin
GO:0071392	cellular response to estradiol stimulus
GO:0071733	transcriptional activation by promoter-enhancer looping
GO:0071899	negative regulation of estrogen receptor binding

Cellular Component

GO:0000812	Swr1 complex
GO:0005622	intracellular
GO:0005634	nucleus
GO:0005719	nuclear euchromatin
GO:0005737	cytoplasm
GO:0030529	ribonucleoprotein complex
GO:0031011	Ino80 complex
GO:0035267	NuA4 histone acetyltransferase complex
GO:0070062	extracellular vesicular exosome
GO:0071339	MLL1 complex

Protein Structure and Domains

PDB ID

MGI:1342299

InterPro

IPR003593 AAA+ ATPase domain
IPR003959 ATPase, AAA-type, core
IPR004504 DNA repair protein RadA
IPR007694 DNA helicase, DnaB-like, C-terminal
IPR010339 TIP49, C-terminal
IPR012340 Nucleic acid-binding, OB-fold
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00004
PF07724
PF13304
PF03796
PF06068

PRINTS

PR01874

PIRSF

SMART

SM00382

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt