InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: WDFY3

Summary

InnateDB Protein

IDBP-27963.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

WDFY3

Protein Name

WD repeat and FYVE domain containing 3

Synonyms

ALFY; ZFYVE25;

Species

Homo sapiens

Ensembl Protein

ENSP00000295888

InnateDB Gene

IDBG-27961 (WDFY3)

Protein Structure

UniProt Annotation

Function

Required for selective autophagy (aggrephagy) but not for autophagic degradation of bulk cytospol in response to starvation. Involved in the formation and degradation of cytoplasmic polyubiquitin-containing bodies (p62 bodies, ALIS/aggresome-like induced structures). May play a role as adaptor or scaffolding protein by promoting the association of the E3-like ligase ATG12-ATG5-ATG16L and LC3 to ubiquitinated target substrate. The association with GABARAP is required for its recruitment to LC3B-positive p62 bodies suggesting a role in targeting certain p62 structures for clearance. Involved in midbody ring degradation. {ECO:0000269PubMed:20168092, ECO:0000269PubMed:20417604, ECO:0000269PubMed:24128730}.

Subcellular Localization

Nucleus membrane. Cytoplasm. Nucleus, PML body. Membrane; Peripheral membrane protein; Cytoplasmic side. Note=Colocalizes with autophagic structures in starved cells. Shuttles between nucleus and cytoplasm; translocation from the nucleus to cytoplasmic polyubiquitin-containing bodies upon cellular stress is dependent on SQSTM1.

Disease Associations

Tissue Specificity

Ubiquitous. Expressed in osteoclast and their mononuclear precursors (at protein level). {ECO:0000269PubMed:15292400, ECO:0000269PubMed:20971078}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 23 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	23 [view]
Protein-Protein	23 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003831	beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity
GO:0005488	binding
GO:0005515	protein binding
GO:0005545	1-phosphatidylinositol binding
GO:0046872	metal ion binding

Biological Process

GO:0016239	positive regulation of macroautophagy
GO:0035973	aggrephagy

Cellular Component

GO:0005635	nuclear envelope
GO:0005737	cytoplasm
GO:0005776	autophagic vacuole
GO:0016234	inclusion body
GO:0016605	PML body
GO:0019898	extrinsic component of membrane
GO:0034274	Atg12-Atg5-Atg16 complex

Protein Structure and Domains

PDB ID

InterPro

IPR000306 FYVE zinc finger
IPR000409 BEACH domain
IPR001680 WD40 repeat
IPR008985 Concanavalin A-like lectin/glucanases superfamily
IPR011011 Zinc finger, FYVE/PHD-type
IPR013763 Cyclin-like
IPR016024 Armadillo-type fold
IPR017455 Zinc finger, FYVE-related
IPR017986 WD40-repeat-containing domain

PFAM

PF01363
PF02138
PF00400

PRINTS

PIRSF

SMART

SM00064
SM01026
SM00320
SM00385

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q8IZQ1