InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: MSH3

Summary

InnateDB Protein

IDBP-31504.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

MSH3

Protein Name

mutS homolog 3 (E. coli)

Synonyms

DUP; MRP1;

Species

Homo sapiens

Ensembl Protein

ENSP00000265081

InnateDB Gene

IDBG-31502 (MSH3)

Protein Structure

UniProt Annotation

Function

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion- deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.

Subcellular Localization

Disease Associations

Endometrial cancer (ENDMC) [MIM:608089]: A malignancy of endometrium, the mucous lining of the uterus. Most endometrial cancers are adenocarcinomas, cancers that begin in cells that make and release mucus and other fluids. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 32 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	32 [view]
Protein-Protein	32 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000403	Y-form DNA binding
GO:0000404	heteroduplex DNA loop binding
GO:0000406	double-strand/single-strand DNA junction binding
GO:0003677	DNA binding
GO:0003684	damaged DNA binding
GO:0003697	single-stranded DNA binding
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008094	DNA-dependent ATPase activity
GO:0019237	centromeric DNA binding
GO:0019899	enzyme binding
GO:0030983	mismatched DNA binding
GO:0032139	dinucleotide insertion or deletion binding
GO:0032142	single guanine insertion binding
GO:0032181	dinucleotide repeat insertion binding
GO:0032357	oxidized purine DNA binding

Biological Process

GO:0000710	meiotic mismatch repair
GO:0006200	ATP catabolic process
GO:0006281	DNA repair
GO:0006298	mismatch repair
GO:0007131	reciprocal meiotic recombination
GO:0016447	somatic recombination of immunoglobulin gene segments
GO:0043570	maintenance of DNA repeat elements
GO:0045910	negative regulation of DNA recombination
GO:0051096	positive regulation of helicase activity

Cellular Component

GO:0000228	nuclear chromosome
GO:0005634	nucleus
GO:0016020	membrane
GO:0032302	MutSbeta complex

Protein Structure and Domains

PDB ID

InterPro

IPR000432 DNA mismatch repair protein MutS, C-terminal
IPR007695 DNA mismatch repair protein MutS-like, N-terminal
IPR007696 DNA mismatch repair protein MutS, core
IPR007860 DNA mismatch repair protein MutS, connector domain
IPR007861 DNA mismatch repair protein MutS, clamp
IPR016151 DNA mismatch repair protein MutS, N-terminal
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00488
PF01624
PF05192
PF05188
PF05190

PRINTS

PIRSF

SMART

SM00534
SM00533

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P20585