InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: EIF3E

Summary

InnateDB Protein

IDBP-32395.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

EIF3E

Protein Name

eukaryotic translation initiation factor 3, subunit E

Synonyms

eIF3-p46; EIF3-P48; EIF3S6; INT6;

Species

Homo sapiens

Ensembl Protein

ENSP00000220849

InnateDB Gene

IDBG-32393 (EIF3E)

Protein Structure

UniProt Annotation

Function

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense- mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins. {ECO:0000255HAMAP-Rule:MF_03004, ECO:0000269PubMed:17310990, ECO:0000269PubMed:17324924, ECO:0000269PubMed:17468741}.

Subcellular Localization

Cytoplasm. Nucleus, PML body.

Disease Associations

Tissue Specificity

Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus. {ECO:0000269PubMed:8688078, ECO:0000269PubMed:9295280}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 145 experimentally validated interaction(s) in this database.
They are also associated with 4 interaction(s) predicted by orthology.

Experimentally validated
Total	145 [view]
Protein-Protein	142 [view]
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	4 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003743	translation initiation factor activity
GO:0005515	protein binding
GO:0044822	poly(A) RNA binding
GO:0047485	protein N-terminus binding

Biological Process

GO:0000184	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
GO:0001731	formation of translation preinitiation complex
GO:0006412	translation
GO:0006413	translational initiation
GO:0006446	regulation of translational initiation
GO:0010467	gene expression
GO:0044267	cellular protein metabolic process
GO:0045947	negative regulation of translational initiation

Cellular Component

GO:0000785	chromatin
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005852	eukaryotic translation initiation factor 3 complex
GO:0016020	membrane
GO:0016282	eukaryotic 43S preinitiation complex
GO:0016605	PML body
GO:0033290	eukaryotic 48S preinitiation complex
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR000717 Proteasome component (PCI) domain
IPR016650 Eukaryotic translation initiation factor 3 subunit E
IPR019010 Eukaryotic translation initiation factor 3 subunit E, N-terminal

PFAM

PF01399
PF09440

PRINTS

PIRSF

PIRSF016255

SMART

SM00088

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt