InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: CENPE

Summary

InnateDB Protein

IDBP-32490.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

CENPE

Protein Name

centromere protein E, 312kDa

Synonyms

CENP-E; KIF10; PPP1R61;

Species

Homo sapiens

Ensembl Protein

ENSP00000265148

InnateDB Gene

IDBG-32488 (CENPE)

Protein Structure

UniProt Annotation

Function

Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end- directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule- dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle. {ECO:0000269PubMed:17535814, ECO:0000269PubMed:7889940}.

Subcellular Localization

Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Associates with kinetochores during congression (as early as prometaphase), relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 28 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	28 [view]
Protein-Protein	24 [view]
Protein-DNA	4 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003700	sequence-specific DNA binding transcription factor activity
GO:0003777	microtubule motor activity
GO:0004871	signal transducer activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0005525	GTP binding
GO:0008017	microtubule binding
GO:0043515	kinetochore binding

Biological Process

GO:0000089	mitotic metaphase
GO:0000278	mitotic cell cycle
GO:0006355	regulation of transcription, DNA-templated
GO:0006614	SRP-dependent cotranslational protein targeting to membrane
GO:0007018	microtubule-based movement
GO:0007079	mitotic chromosome movement towards spindle pole
GO:0007080	mitotic metaphase plate congression
GO:0007165	signal transduction
GO:0007275	multicellular organismal development
GO:0007596	blood coagulation
GO:0008152	metabolic process
GO:0019886	antigen processing and presentation of exogenous peptide antigen via MHC class II
GO:0045860	positive regulation of protein kinase activity
GO:0051382	kinetochore assembly

Cellular Component

GO:0000775	chromosome, centromeric region
GO:0000776	kinetochore
GO:0000778	condensed nuclear chromosome kinetochore
GO:0000779	condensed chromosome, centromeric region
GO:0000940	condensed chromosome outer kinetochore
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005819	spindle
GO:0005829	cytosol
GO:0005871	kinesin complex
GO:0005874	microtubule
GO:0015630	microtubule cytoskeleton
GO:0016020	membrane

Protein Structure and Domains

PDB ID

InterPro

IPR001752 Kinesin, motor domain
IPR013822 Signal recognition particle, SRP54 subunit, helical bundle
IPR015988 STAT transcription factor, coiled coil
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00225
PF02881

PRINTS

PR00380

PIRSF

SMART

SM00129
SM00963

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt