InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: TOP3A

Summary

InnateDB Protein

IDBP-33853.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

TOP3A

Protein Name

topoisomerase (DNA) III alpha

Synonyms

TOP3; ZGRF7;

Species

Homo sapiens

Ensembl Protein

ENSP00000321636

InnateDB Gene

IDBG-33849 (TOP3A)

Protein Structure

UniProt Annotation

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity. {ECO:0000269PubMed:20445207, ECO:0000269PubMed:8622991}.

Subcellular Localization

Disease Associations

Tissue Specificity

High expression is found in testis, heart, skeletal muscle and pancreas.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 36 experimentally validated interaction(s) in this database.

Experimentally validated
Total	36 [view]
Protein-Protein	34 [view]
Protein-DNA	2 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003916	DNA topoisomerase activity
GO:0003917	DNA topoisomerase type I activity
GO:0005515	protein binding
GO:0008270	zinc ion binding

Biological Process

GO:0006265	DNA topological change
GO:0007126	meiotic nuclear division

Cellular Component

GO:0005634	nucleus
GO:0005694	chromosome
GO:0016605	PML body

Protein Structure and Domains

PDB ID

InterPro

IPR000380 DNA topoisomerase, type IA
IPR001878 Zinc finger, CCHC-type
IPR003601 DNA topoisomerase, type IA, domain 2
IPR003602 DNA topoisomerase, type IA, DNA-binding domain
IPR006171 Toprim domain
IPR010666 Zinc finger, GRF-type
IPR013497 DNA topoisomerase, type IA, central
IPR013498 DNA topoisomerase, type IA, zn finger
IPR023405 DNA topoisomerase, type IA, core domain

PFAM

PF00098
PF01751
PF13662
PF06839
PF01131
PF01396

PRINTS

PR00417

PIRSF

SMART

SM00343
SM00436
SM00437
SM00493

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt