InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: SGOL1

Summary

InnateDB Protein

IDBP-367482.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

SGOL1

Protein Name

shugoshin-like 1 (S. pombe)

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000411200

InnateDB Gene

IDBG-21863 (SGOL1)

Protein Structure

UniProt Annotation

Function

Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. {ECO:0000269PubMed:15604152, ECO:0000269PubMed:15723797, ECO:0000269PubMed:15737064, ECO:0000269PubMed:16580887, ECO:0000269PubMed:17617734, ECO:0000269PubMed:17621308, ECO:0000269PubMed:18331714}.

Subcellular Localization

Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase. During prometaphase, it localizes to a single focus, while at metaphase, it localizes to 2 spots corresponding to the 2 centromeres. Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A. Localizes to the inner kinetochore from prophase to early metaphase. Colocalizes with NEK2 and SS18L1 at the kinetochore. Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore. Isoform 1 primarily localizes to kinetochores during G2 phase and mitotic prophase, metaphase, and anaphase and does not appear to be associated with kinetochores during late mitosis. Isoform 3 is found at the centrosome in interphase and at spindle poles in mitosis and its spindle pole localization is PLK1 dependent. Isoform 3 does not localize to kinetochores during any stages of the cell cycle.

Disease Associations

Tissue Specificity

Widely expressed. Highly expressed in testis. Expressed in lung, small intestine, breast, liver and placenta. Strongly overexpressed in 90% of breast cancers tested. {ECO:0000269PubMed:12747765}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 36 experimentally validated interaction(s) in this database.

Experimentally validated
Total	36 [view]
Protein-Protein	35 [view]
Protein-DNA	0
Protein-RNA	1 [view]
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0019900	kinase binding

Biological Process

GO:0000278	mitotic cell cycle
GO:0007059	chromosome segregation
GO:0007067	mitotic nuclear division
GO:0008608	attachment of spindle microtubules to kinetochore
GO:0010457	centriole-centriole cohesion
GO:0045132	meiotic chromosome segregation

Cellular Component

GO:0000775	chromosome, centromeric region
GO:0000776	kinetochore
GO:0000777	condensed chromosome kinetochore
GO:0000779	condensed chromosome, centromeric region
GO:0000922	spindle pole
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005813	centrosome
GO:0005829	cytosol
GO:0030892	mitotic cohesin complex