InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: SPAG5

Summary

InnateDB Protein

IDBP-36957.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

SPAG5

Protein Name

sperm associated antigen 5

Synonyms

DEEPEST; hMAP126; MAP126;

Species

Homo sapiens

Ensembl Protein

ENSP00000323300

InnateDB Gene

IDBG-36955 (SPAG5)

Protein Structure

UniProt Annotation

Function

Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. In complex with SKAP, promotes stable microtubule- kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes. In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation- induced apoptosis. May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU. {ECO:0000269PubMed:11724960, ECO:0000269PubMed:12356910, ECO:0000269PubMed:17664331, ECO:0000269PubMed:18055457, ECO:0000269PubMed:21402792, ECO:0000269PubMed:23953116}.

Subcellular Localization

Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic granule. Note=In a punctate pattern in interphase cells. During mitosis, detected at spindle poles during prophase, throughout the spindle in metaphase and anaphase, and at midzone microtubules in anaphase and telophase. Efficient targeting to the mitotic spindle may depend upon phosphorylation by GSK3B. Detected on kinetochores of chromosomes that have congressed. The astrin (SPAG5)-kinastrin (SKAP) complex localizes to the microtubule plus ends (By similarity). In non-mitotic non-stressed cells, shows a microtubuli pattern. In arsenite-stressed cells, accumulates in stress granules. {ECO:0000250}.

Disease Associations

Tissue Specificity

Highly expressed in testis. Detected at low levels in placenta, liver, pancreas, thymus and colon. {ECO:0000269PubMed:11549262}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 31 experimentally validated interaction(s) in this database.

Experimentally validated
Total	31 [view]
Protein-Protein	31 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding

Biological Process

GO:0000070	mitotic sister chromatid segregation
GO:0007049	cell cycle
GO:0007051	spindle organization
GO:0007059	chromosome segregation
GO:0048015	phosphatidylinositol-mediated signaling
GO:0051988	regulation of attachment of spindle microtubules to kinetochore

Cellular Component

GO:0000776	kinetochore
GO:0000777	condensed chromosome kinetochore
GO:0000922	spindle pole
GO:0005737	cytoplasm
GO:0005815	microtubule organizing center
GO:0030496	midbody
GO:0035371	microtubule plus-end
GO:0072686	mitotic spindle