InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: SUPT5H

Summary

InnateDB Protein

IDBP-379873.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

SUPT5H

Protein Name

suppressor of Ty 5 homolog (S. cerevisiae)

Synonyms

SPT5; SPT5H; Tat-CT1;

Species

Homo sapiens

Ensembl Protein

ENSP00000404029

InnateDB Gene

IDBG-50457 (SUPT5H)

Protein Structure

UniProt Annotation

Function

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV- 1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. {ECO:0000269PubMed:10075709, ECO:0000269PubMed:10199401, ECO:0000269PubMed:10393184, ECO:0000269PubMed:10421630, ECO:0000269PubMed:10454543, ECO:0000269PubMed:10757782, ECO:0000269PubMed:10912001, ECO:0000269PubMed:11112772, ECO:0000269PubMed:11553615, ECO:0000269PubMed:11809800, ECO:0000269PubMed:12653964, ECO:0000269PubMed:12718890, ECO:0000269PubMed:14701750, ECO:0000269PubMed:15136722, ECO:0000269PubMed:15380072, ECO:0000269PubMed:16214896, ECO:0000269PubMed:9450929, ECO:0000269PubMed:9514752, ECO:0000269PubMed:9857195}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:10075709}.

Disease Associations

Tissue Specificity

Ubiquitously expressed. {ECO:0000269PubMed:10075709, ECO:0000269PubMed:8975720}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 86 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	86 [view]
Protein-Protein	79 [view]
Protein-DNA	6 [view]
Protein-RNA	0
DNA-DNA	1 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0019899	enzyme binding
GO:0044822	poly(A) RNA binding
GO:0046982	protein heterodimerization activity

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0006338	chromatin remodeling
GO:0006354	DNA-dependent transcription, elongation
GO:0006355	regulation of transcription, DNA-templated
GO:0006357	regulation of transcription from RNA polymerase II promoter
GO:0006366	transcription from RNA polymerase II promoter
GO:0006368	transcription elongation from RNA polymerase II promoter
GO:0006370	7-methylguanosine mRNA capping
GO:0007049	cell cycle
GO:0010033	response to organic substance
GO:0010467	gene expression
GO:0016032	viral process
GO:0016239	positive regulation of macroautophagy
GO:0032784	regulation of DNA-templated transcription, elongation
GO:0032785	negative regulation of DNA-dependent transcription, elongation
GO:0032786	positive regulation of DNA-templated transcription, elongation
GO:0039692	single stranded viral RNA replication via double stranded DNA intermediate
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0050434	positive regulation of viral transcription

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0032044	DSIF complex

Protein Structure and Domains

PDB ID

InterPro

IPR005100 Transcription elongation factor Spt5, NGN domain
IPR005824 KOW
IPR006645 NusG, N-terminal
IPR008991 Translation protein SH3-like domain
IPR017071 Transcription elongation factor Spt5
IPR022581 Spt5 transcription elongation factor, N-terminal

PFAM

PF03439
PF00467
PF02357
PF11942

PRINTS

PIRSF

PIRSF036945

SMART

SM00739
SM00738

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt