InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: TLK1

Summary

InnateDB Protein

IDBP-387464.4

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

TLK1

Protein Name

tousled-like kinase 1

Synonyms

PKU-beta;

Species

Homo sapiens

Ensembl Protein

ENSP00000411099

InnateDB Gene

IDBG-74812 (TLK1)

Protein Structure

UniProt Annotation

Function

Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S- phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'. {ECO:0000269PubMed:10523312, ECO:0000269PubMed:10588641, ECO:0000269PubMed:11314006, ECO:0000269PubMed:11470414, ECO:0000269PubMed:12660173, ECO:0000269PubMed:9427565}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:10523312, ECO:0000269PubMed:10588641, ECO:0000269PubMed:11314006, ECO:0000269PubMed:9427565}.

Disease Associations

Tissue Specificity

Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined. {ECO:0000269PubMed:10588641, ECO:0000269PubMed:9427565}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 19 experimentally validated interaction(s) in this database.

Experimentally validated
Total	19 [view]
Protein-Protein	18 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups

Biological Process

GO:0001672	regulation of chromatin assembly or disassembly
GO:0006468	protein phosphorylation
GO:0006886	intracellular protein transport
GO:0006974	cellular response to DNA damage stimulus
GO:0007049	cell cycle
GO:0016568	chromatin modification
GO:0035556	intracellular signal transduction

Cellular Component

GO:0005634

nucleus

Protein Structure and Domains

PDB ID

InterPro

IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM

PF00069
PF07714

PRINTS

PR00109

PIRSF

SMART

SM00220
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt