InnateDB Protein
|
IDBP-388482.5
|
Last Modified
|
2014-10-13 [Report errors or provide feedback]
|
Gene Symbol
|
PIP5K1A
|
Protein Name
|
phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
|
Synonyms
|
|
Species
|
Homo sapiens
|
Ensembl Protein
|
ENSP00000415648
|
InnateDB Gene
|
IDBG-102321 (PIP5K1A)
|
Protein Structure
|
|
Function |
Catalyzes the phosphorylation of phosphatidylinositol 4- phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. {ECO:0000269PubMed:18288197, ECO:0000269PubMed:19158393, ECO:0000269PubMed:20660631}.
|
Subcellular Localization |
Cell membrane. Cytoplasm {ECO:0000250}. Nucleus speckle. Cell projection, ruffle. Note=Colocalizes with RAC1 at actin-rich membrane ruffles. Localizes to nuclear speckles and associates with TUT1 to regulate polyadenylation of selected mRNAs.
|
Disease Associations |
|
Tissue Specificity |
Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver.
|
Comments |
|
Number of Interactions
|
This gene and/or its encoded proteins are associated with 14 experimentally validated interaction(s) in this database.
Experimentally validated |
Total |
14
[view]
|
Protein-Protein |
12
[view]
|
Protein-DNA |
2
[view]
|
Protein-RNA |
0
|
DNA-DNA |
0
|
RNA-RNA |
0
|
DNA-RNA |
0
|
|
|
|
Molecular Function |
|
Biological Process |
|
Cellular Component |
|
PDB ID |
|
InterPro |
IPR002498
Phosphatidylinositol-4-phosphate 5-kinase, core
IPR016034
Phosphatidylinositol-4-phosphate 5-kinase, core, subgroup
|
PFAM |
PF01504
|
PRINTS |
|
PIRSF |
|
SMART |
SM00330
|
TIGRFAMs |
|
Modification |
|
SwissProt |
Q99755
|
PhosphoSite |
PhosphoSite-Q99755
|
TrEMBL |
|
UniProt Splice Variant |
|
Entrez Gene |
8394
|
UniGene |
Hs.731305
|
RefSeq |
NP_001129108
|
HUGO |
HGNC:8994
|
OMIM |
603275
|
CCDS |
CCDS44220
|
HPRD |
|
IMGT |
|
EMBL |
AK291015
AK295691
AL592424
BC007833
CH471121
U78575
U78576
U78577
|
GenPept |
AAC50910
AAC50911
AAC50912
AAH07833
BAF83704
BAG58542
CAI16386
CAI16387
CAI16388
EAW53461
|
|
|