InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Mlkl

Summary

InnateDB Protein

IDBP-400455.4

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Mlkl

Protein Name

mixed lineage kinase domain-like

Synonyms

9130019I15Rik;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000113718

InnateDB Gene

IDBG-193153 (Mlkl)

Protein Structure

UniProt Annotation

Function

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. {ECO:0000269PubMed:23835476, ECO:0000269PubMed:24012422, ECO:0000269PubMed:24019532}.

Subcellular Localization

Cytoplasm {ECO:0000250}. Cell membrane {ECO:0000250}. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction. {ECO:0000250}.

Disease Associations

Tissue Specificity

Highly expressed in thymus, colon, intestine, liver, spleen and lung. Expressed at much lower level in skeletal muscle, heart and kidney. Not detected in brain. {ECO:0000269PubMed:23835476}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 3 experimentally validated interaction(s) in this database.
They are also associated with 12 interaction(s) predicted by orthology.

Experimentally validated
Total	3 [view]
Protein-Protein	2 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	12 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0019901	protein kinase binding
GO:0032403	protein complex binding

Biological Process

GO:0006468	protein phosphorylation
GO:0070207	protein homotrimerization
GO:0070266	necroptotic process

Cellular Component

GO:0005737	cytoplasm
GO:0005886	plasma membrane

Protein Structure and Domains

PDB ID

MGI:1921818

InterPro

IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM

PF00069
PF07714

PRINTS

PR00109

PIRSF

SMART

SM00220
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt