Version 5.4
Mus musculus Protein: Klhl22
Summary
InnateDB Protein IDBP-401555.4
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol Klhl22
Protein Name kelch-like 22 (Drosophila)
Synonyms
Species Mus musculus
Ensembl Protein ENSMUSP00000112412
InnateDB Gene IDBG-140145 (Klhl22)
Protein Structure
UniProt Annotation
Function Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation (By similarity). {ECO:0000250}.
Subcellular Localization Cytoplasm {ECO:0000250}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}. Cytoplasm, cytoskeleton, spindle {ECO:0000250}. Note=Mainly. cytoplasmic in prophase and prometaphase. Associates with the mitotic spindle as the cells reach chromosome bi- orientation. Localizes to the centrosomes shortly before cells enter anaphase After anaphase onset, predominantly associates with the polar microtubules connecting the 2 opposing centrosomes and gradually diffuses into the cytoplasm during telophase (By similarity). {ECO:0000250}.
Disease Associations
Tissue Specificity
Comments
Interactions
Number of Interactions This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 20 interaction(s) predicted by orthology.
Predicted by orthology
Total 20 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0003674 molecular_function
GO:0005515 protein binding
Biological Process
GO:0000070 mitotic sister chromatid segregation
GO:0006513 protein monoubiquitination
GO:0007094 mitotic spindle assembly checkpoint
GO:0051301 cell division
Cellular Component
GO:0005737 cytoplasm
GO:0005813 centrosome
GO:0005827 polar microtubule
GO:0031463 Cul3-RING ubiquitin ligase complex
GO:0072686 mitotic spindle
Protein Structure and Domains
PDB ID MGI:1337995
InterPro IPR000210 BTB/POZ-like
IPR006652 Kelch repeat type 1
IPR011333 BTB/POZ fold
IPR011498 Kelch repeat type 2
IPR011705 BTB/Kelch-associated
IPR013069 BTB/POZ
IPR017096 Kelch-like protein, gigaxonin type
PFAM PF01344
PF07646
PF07707
PF00651
PRINTS
PIRSF PIRSF037037
SMART SM00225
SM00612
SM00875
TIGRFAMs
Post-translational Modifications
Modification
Cross-References
SwissProt Q99JN2
PhosphoSite PhosphoSite-Q99JN2
TrEMBL D3Z7N9
UniProt Splice Variant
Entrez Gene 224023
UniGene Mm.282807
RefSeq XP_006522104
MGI ID
MGI Symbol Klhl22
OMIM
CCDS CCDS49783
HPRD
IMGT
EMBL AC087802 AK028197 AK143514 BC005800
GenPept AAH05800 BAC25806 BAE25409

If you use InnateDB for your research, please cite the following publication:

Breuer et al., InnateDB: systems biology of innate immunity and beyond - recent updates and continuing curation. Nucl. Acids Res. (2013) 41 (D1) 

InnateDB is being developed jointly by the Brinkman Laboratory (Simon Fraser University, British Columbia, Canada), the Hancock Laboratory (University of British Columbia, Vancouver, British Columbia) and the Lynn EMBL Australia Group (South Australian Health & Medical Research Institute and Flinders University, Adelaide, Australia).

Funding is currently provided by Allergen and EMBL Australia. Previous funding has been provided by Genome Canada, the Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative and by Teagasc. InnateDB curated interactions are licensed under the Design Science License. All other data is licensed under the terms of the originating database. Contact: innatedb-mail@sfu.ca