InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: AGAP2

Summary

InnateDB Protein

IDBP-43542.7

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

AGAP2

Protein Name

ArfGAP with GTPase domain, ankyrin repeat and PH domain 2

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000257897

InnateDB Gene

IDBG-43540 (AGAP2)

Protein Structure

UniProt Annotation

Function

GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. {ECO:0000269PubMed:12640130, ECO:0000269PubMed:14761976, ECO:0000269PubMed:15118108, ECO:0000269PubMed:16079295}.

Subcellular Localization

Isoform 1: Cytoplasm. Nucleus.Isoform 2: Cytoplasm.

Disease Associations

Tissue Specificity

Isoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart. {ECO:0000269PubMed:12640130, ECO:0000269PubMed:14761976, ECO:0000269PubMed:15118108, ECO:0000269PubMed:16079295, ECO:0000269PubMed:16150119}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 15 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	15 [view]
Protein-Protein	15 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0005525	GTP binding
GO:0008060	ARF GTPase activator activity
GO:0008270	zinc ion binding

Biological Process

GO:0006184	GTP catabolic process
GO:0007165	signal transduction
GO:0007264	small GTPase mediated signal transduction
GO:0007411	axon guidance
GO:0015031	protein transport
GO:0032312	regulation of ARF GTPase activity
GO:0042177	negative regulation of protein catabolic process
GO:0043524	negative regulation of neuron apoptotic process
GO:0043547	positive regulation of GTPase activity

Cellular Component

GO:0005622	intracellular
GO:0005634	nucleus
GO:0005730	nucleolus
GO:0005739	mitochondrion
GO:0016020	membrane

Protein Structure and Domains

PDB ID

InterPro

IPR001164 Arf GTPase activating protein
IPR001806 Small GTPase superfamily
IPR001849 Pleckstrin homology domain
IPR002110 Ankyrin repeat
IPR003579 Small GTPase superfamily, Rab type
IPR013684 Mitochondrial Rho-like
IPR020683 Ankyrin repeat-containing domain
IPR020849 Small GTPase superfamily, Ras type
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM