Homo sapiens Protein: DAPK1 | |||||||||||||||||||||||||||
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Summary | |||||||||||||||||||||||||||
InnateDB Protein | IDBP-478941.3 | ||||||||||||||||||||||||||
Last Modified | 2014-10-13 [Report errors or provide feedback] | ||||||||||||||||||||||||||
Gene Symbol | DAPK1 | ||||||||||||||||||||||||||
Protein Name | death-associated protein kinase 1 | ||||||||||||||||||||||||||
Synonyms | DAPK; | ||||||||||||||||||||||||||
Species | Homo sapiens | ||||||||||||||||||||||||||
Ensembl Protein | ENSP00000417076 | ||||||||||||||||||||||||||
InnateDB Gene | IDBG-74918 (DAPK1) | ||||||||||||||||||||||||||
Protein Structure | |||||||||||||||||||||||||||
UniProt Annotation | |||||||||||||||||||||||||||
Function | Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript- selective translation inhibition.Isoform 2 cannot induce apoptosis but can induce membrane blebbing. | ||||||||||||||||||||||||||
Subcellular Localization | Isoform 1: Cytoplasm. Cytoplasm, cytoskeleton. Note=Colocalizes with MAP1B in the microtubules and cortical actin fibers.Isoform 2: Cytoplasm. Cytoplasm, cytoskeleton. | ||||||||||||||||||||||||||
Disease Associations | |||||||||||||||||||||||||||
Tissue Specificity | Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas. {ECO:0000269PubMed:18422656}. | ||||||||||||||||||||||||||
Comments | |||||||||||||||||||||||||||
Interactions | |||||||||||||||||||||||||||
Number of Interactions |
This gene and/or its encoded proteins are associated with 51 experimentally validated interaction(s) in this database.
They are also associated with 4 interaction(s) predicted by orthology.
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Gene Ontology | |||||||||||||||||||||||||||
Molecular Function |
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Biological Process |
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Cellular Component |
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Protein Structure and Domains | |||||||||||||||||||||||||||
PDB ID | |||||||||||||||||||||||||||
InterPro |
IPR000488
Death domain IPR000719 Protein kinase domain IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain IPR002110 Ankyrin repeat IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain IPR010440 Lipopolysaccharide kinase IPR011009 Protein kinase-like domain IPR011029 Death-like domain IPR018934 RIO-like kinase IPR020635 Tyrosine-protein kinase, catalytic domain IPR020683 Ankyrin repeat-containing domain IPR027417 P-loop containing nucleoside triphosphate hydrolase |
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PFAM |
PF00531
PF00069 PF07714 PF00023 PF13606 PF06293 PF01163 PF11929 PF12796 |
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PRINTS |
PR00109
PR01415 |
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PIRSF | |||||||||||||||||||||||||||
SMART |
SM00005
SM00248 SM00220 SM00219 |
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TIGRFAMs | |||||||||||||||||||||||||||
Post-translational Modifications | |||||||||||||||||||||||||||
Modification | |||||||||||||||||||||||||||
Cross-References | |||||||||||||||||||||||||||
SwissProt | P53355 | ||||||||||||||||||||||||||
PhosphoSite | PhosphoSite-P53355 | ||||||||||||||||||||||||||
TrEMBL | |||||||||||||||||||||||||||
UniProt Splice Variant | |||||||||||||||||||||||||||
Entrez Gene | 1612 | ||||||||||||||||||||||||||
UniGene | Hs.380277 | ||||||||||||||||||||||||||
RefSeq | NP_001275659 | ||||||||||||||||||||||||||
HUGO | HGNC:2674 | ||||||||||||||||||||||||||
OMIM | 600831 | ||||||||||||||||||||||||||
CCDS | CCDS43842 | ||||||||||||||||||||||||||
HPRD | 02902 | ||||||||||||||||||||||||||
IMGT | |||||||||||||||||||||||||||
EMBL | AK127855 AL160279 AL161787 AL591852 BC113660 BC143733 BC143759 BT006935 CH471089 CR749834 DQ436495 X76104 | ||||||||||||||||||||||||||
GenPept | AAI13661 AAI43734 AAI43760 AAP35581 ABD96827 BAC87163 CAA53712 CAH18690 EAW62727 | ||||||||||||||||||||||||||