InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: NOS3

Summary

InnateDB Protein

IDBP-48573.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

NOS3

Protein Name

nitric oxide synthase 3 (endothelial cell)

Synonyms

ECNOS; eNOS;

Species

Homo sapiens

Ensembl Protein

ENSP00000297494

InnateDB Gene

IDBG-48571 (NOS3)

Protein Structure

UniProt Annotation

Function

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Subcellular Localization

Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.

Disease Associations

Tissue Specificity

Platelets, placenta, liver and kidney. {ECO:0000269PubMed:7515611}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 69 experimentally validated interaction(s) in this database.

Experimentally validated
Total	69 [view]
Protein-Protein	69 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003785	actin monomer binding
GO:0003958	NADPH-hemoprotein reductase activity
GO:0004517	nitric-oxide synthase activity
GO:0005506	iron ion binding
GO:0005515	protein binding
GO:0005516	calmodulin binding
GO:0010181	FMN binding
GO:0016491	oxidoreductase activity
GO:0020037	heme binding
GO:0034617	tetrahydrobiopterin binding
GO:0034618	arginine binding
GO:0046870	cadmium ion binding
GO:0050660	flavin adenine dinucleotide binding
GO:0050661	NADP binding

Biological Process

GO:0001525	angiogenesis
GO:0001542	ovulation from ovarian follicle
GO:0001701	in utero embryonic development
GO:0001974	blood vessel remodeling
GO:0002028	regulation of sodium ion transport
GO:0003057	regulation of the force of heart contraction by chemical signal
GO:0003100	regulation of systemic arterial blood pressure by endothelin
GO:0006527	arginine catabolic process
GO:0006809	nitric oxide biosynthetic process
GO:0007005	mitochondrion organization
GO:0007263	nitric oxide mediated signal transduction
GO:0007596	blood coagulation
GO:0008217	regulation of blood pressure
GO:0008285	negative regulation of cell proliferation
GO:0009408	response to heat
GO:0010544	negative regulation of platelet activation
GO:0014740	negative regulation of muscle hyperplasia
GO:0014806	smooth muscle hyperplasia
GO:0030324	lung development
GO:0031284	positive regulation of guanylate cyclase activity
GO:0031663	lipopolysaccharide-mediated signaling pathway
GO:0034405	response to fluid shear stress
GO:0043267	negative regulation of potassium ion transport
GO:0043542	endothelial cell migration
GO:0044281	small molecule metabolic process
GO:0045766	positive regulation of angiogenesis
GO:0045776	negative regulation of blood pressure
GO:0045909	positive regulation of vasodilation
GO:0046209	nitric oxide metabolic process
GO:0050880	regulation of blood vessel size
GO:0050999	regulation of nitric-oxide synthase activity
GO:0051346	negative regulation of hydrolase activity
GO:0051701	interaction with host
GO:0051926	negative regulation of calcium ion transport
GO:0055114	oxidation-reduction process
GO:0090382	phagosome maturation
GO:1902042	negative regulation of extrinsic apoptotic signaling pathway via death domain receptors

Cellular Component

GO:0000139	Golgi membrane
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005856	cytoskeleton
GO:0005886	plasma membrane
GO:0005901	caveola
GO:0030666	endocytic vesicle membrane

Protein Structure and Domains

PDB ID

InterPro

IPR001094 Flavodoxin
IPR001433 Oxidoreductase FAD/NAD(P)-binding
IPR001709 Flavoprotein pyridine nucleotide cytochrome reductase
IPR003097 FAD-binding, type 1
IPR004030 Nitric oxide synthase, N-terminal
IPR008254 Flavodoxin/nitric oxide synthase
IPR012144 Nitric-oxide synthase, eukaryote
IPR017938 Riboflavin synthase-like beta-barrel
IPR029039 Flavoprotein-like

PFAM

PF00175
PF00667
PF02898
PF00258

PRINTS

PR00369
PR00371

PIRSF

PIRSF000333

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt