InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: PRPF19

Summary

InnateDB Protein

IDBP-49438.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

PRPF19

Protein Name

PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000227524

InnateDB Gene

IDBG-49436 (PRPF19)

Protein Structure

UniProt Annotation

Function

Plays a role in DNA double-strand break (DSB) repair. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. {ECO:0000269PubMed:11082287, ECO:0000269PubMed:12960389, ECO:0000269PubMed:15660529, ECO:0000269PubMed:16223718, ECO:0000269PubMed:16332694, ECO:0000269PubMed:16388800}.

Subcellular Localization

Nucleus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, spindle. Note=Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin.

Disease Associations

Tissue Specificity

Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines. {ECO:0000269PubMed:10404385, ECO:0000269PubMed:11082287, ECO:0000269PubMed:12960389, ECO:0000269PubMed:16388800}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 151 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	151 [view]
Protein-Protein	151 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0034450	ubiquitin-ubiquitin ligase activity
GO:0042802	identical protein binding

Biological Process

GO:0000209	protein polyubiquitination
GO:0000245	spliceosomal complex assembly
GO:0000398	mRNA splicing, via spliceosome
GO:0006281	DNA repair
GO:0016567	protein ubiquitination

Cellular Component

GO:0000151	ubiquitin ligase complex
GO:0000974	Prp19 complex
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005819	spindle
GO:0016020	membrane
GO:0016607	nuclear speck
GO:0071013	catalytic step 2 spliceosome

Protein Structure and Domains

PDB ID

InterPro

IPR000772 Ricin B lectin domain
IPR001680 WD40 repeat
IPR003613 U box domain
IPR013915 Pre-mRNA-splicing factor 19
IPR017986 WD40-repeat-containing domain
IPR020472 G-protein beta WD-40 repeat

PFAM

PF00652
PF14200
PF00400
PF04564
PF08606

PRINTS

PR00320

PIRSF

SMART

SM00458
SM00320
SM00504

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt