InnateDB Protein
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IDBP-50232.5
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Last Modified
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2014-10-13 [Report errors or provide feedback]
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Gene Symbol
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PAF1
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Protein Name
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Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae)
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Synonyms
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Species
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Homo sapiens
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Ensembl Protein
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ENSP00000221265
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InnateDB Gene
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IDBG-50230 (PAF1)
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Protein Structure
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Function |
Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors. Has oncogenic activity in vivo and in vitro. {ECO:0000269PubMed:16491129, ECO:0000269PubMed:19410543, ECO:0000269PubMed:19952111, ECO:0000269PubMed:20178742, ECO:0000269PubMed:20541477, ECO:0000269PubMed:21329879, ECO:0000269PubMed:22419161}.
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Subcellular Localization |
Nucleus {ECO:0000269PubMed:16491129}. Note=Punctuate distribution throughout the nucleus except in nucleoli and the perinuclear chromatin.
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Disease Associations |
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Tissue Specificity |
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Comments |
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Number of Interactions
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This gene and/or its encoded proteins are associated with 75 experimentally validated interaction(s) in this database.
They are also associated with 5 interaction(s) predicted by orthology.
Experimentally validated |
Total |
75
[view]
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Protein-Protein |
70
[view]
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Protein-DNA |
5
[view]
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Protein-RNA |
0
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DNA-DNA |
0
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RNA-RNA |
0
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DNA-RNA |
0
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Predicted by orthology |
Total |
5 [view]
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Molecular Function |
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Biological Process |
GO:0000122
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negative regulation of transcription from RNA polymerase II promoter
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GO:0001711
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endodermal cell fate commitment
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GO:0006351
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transcription, DNA-templated
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GO:0006378
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mRNA polyadenylation
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GO:0010390
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histone monoubiquitination
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GO:0016055
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Wnt signaling pathway
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GO:0019827
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stem cell maintenance
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GO:0031442
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positive regulation of mRNA 3'-end processing
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GO:0032968
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positive regulation of transcription elongation from RNA polymerase II promoter
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GO:0033523
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histone H2B ubiquitination
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GO:0045638
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negative regulation of myeloid cell differentiation
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GO:0045944
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positive regulation of transcription from RNA polymerase II promoter
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GO:0071222
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cellular response to lipopolysaccharide
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Cellular Component |
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PDB ID |
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InterPro |
IPR007133
RNA polymerase II-associated, Paf1
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PFAM |
PF03985
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PRINTS |
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PIRSF |
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SMART |
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TIGRFAMs |
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Modification |
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SwissProt |
Q8N7H5
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PhosphoSite |
PhosphoSite-Q8N7H5
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TrEMBL |
A0A024R0H6
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UniProt Splice Variant |
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Entrez Gene |
54623
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UniGene |
Hs.466714
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RefSeq |
NP_061961
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HUGO |
HGNC:25459
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OMIM |
610506
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CCDS |
CCDS12533
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HPRD |
10140
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IMGT |
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EMBL |
AC005239
AJ401156
AK001985
AK098423
BC000017
BC013402
CH471126
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GenPept |
AAC25503
AAH00017
AAH13402
BAA92020
BAC05305
CAC20564
EAW56880
EAW56881
EAW56882
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