InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Runx2

Summary

InnateDB Protein

IDBP-532060.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Runx2

Protein Name

runt related transcription factor 2

Synonyms

AML3; Cbf; Cbfa-1; Cbfa1; LS3; Osf2; Pebp2a1; PEBP2aA; Pebpa2a;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000123743

InnateDB Gene

IDBG-184938 (Runx2)

Protein Structure

UniProt Annotation

Function

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'- PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. Inhibits KAT6B-dependent transcriptional activation (By similarity). In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF- responsive element (OCFRE). {ECO:0000250, ECO:0000269PubMed:20484411, ECO:0000269PubMed:9182763}.

Subcellular Localization

Nucleus.

Disease Associations

Tissue Specificity

Found in thymus and testis, T-cell lines but not in B-cell lines. Isoform 2 is exclusively found in bone, particularly in osteoblasts; isoforms 3 and 4 are expressed in T- cell lines; isoforms 5, 6, 7, 8 and 9 can be found in osteoblasts and osteosarcoma cell lines.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 37 experimentally validated interaction(s) in this database.
They are also associated with 46 interaction(s) predicted by orthology.

Experimentally validated
Total	37 [view]
Protein-Protein	32 [view]
Protein-DNA	5 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	46 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000978	RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0001077	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription
GO:0003677	DNA binding
GO:0003682	chromatin binding
GO:0003700	sequence-specific DNA binding transcription factor activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0019904	protein domain specific binding
GO:0043425	bHLH transcription factor binding
GO:0044212	transcription regulatory region DNA binding
GO:0070491	repressing transcription factor binding

Biological Process

GO:0001501	skeletal system development
GO:0001649	osteoblast differentiation
GO:0001958	endochondral ossification
GO:0002051	osteoblast fate commitment
GO:0002062	chondrocyte differentiation
GO:0002063	chondrocyte development
GO:0002076	osteoblast development
GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0008284	positive regulation of cell proliferation
GO:0030217	T cell differentiation
GO:0030278	regulation of ossification
GO:0030509	BMP signaling pathway
GO:0032332	positive regulation of chondrocyte differentiation
GO:0035115	embryonic forelimb morphogenesis
GO:0040036	regulation of fibroblast growth factor receptor signaling pathway
GO:0042475	odontogenesis of dentin-containing tooth
GO:0042487	regulation of odontogenesis of dentin-containing tooth
GO:0045667	regulation of osteoblast differentiation
GO:0045669	positive regulation of osteoblast differentiation
GO:0045879	negative regulation of smoothened signaling pathway
GO:0045892	negative regulation of transcription, DNA-templated
GO:0045893	positive regulation of transcription, DNA-templated
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0048469	cell maturation
GO:0048701	embryonic cranial skeleton morphogenesis
GO:0048705	skeletal system morphogenesis
GO:0048863	stem cell differentiation
GO:0071773	cellular response to BMP stimulus
GO:1901522	positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus

Cellular Component

GO:0000790	nuclear chromatin
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005667	transcription factor complex
GO:0005737	cytoplasm

Protein Structure and Domains

PDB ID

MGI:99829

InterPro

IPR000040 Acute myeloid leukemia 1 protein (AML1)/Runt
IPR008967 p53-like transcription factor, DNA-binding
IPR013524 Runt domain
IPR013711 Runx, C-terminal domain

PFAM

PF00853
PF08504

PRINTS

PR00967

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q08775