InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Enpp1

Summary

InnateDB Protein

IDBP-534250.4

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Enpp1

Protein Name

ectonucleotide pyrophosphatase/phosphodiesterase 1

Synonyms

4833416E15Rik; AI428932; C76301; CD203c; E-NPP1; Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw; twy;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000114273

InnateDB Gene

IDBG-138688 (Enpp1)

Protein Structure

UniProt Annotation

Function

Appears to modulate insulin sensitivity (By similarity). By generating PPi, plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. PPi inhibits mineralization by binding to nascent hydroxyapatite (HA) crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. {ECO:0000250, ECO:0000269PubMed:1647027, ECO:0000269PubMed:9662402}.

Subcellular Localization

Cell membrane; Single-pass type II membrane protein. Basolateral cell membrane; Single-pass type II membrane protein. Secreted. Note=Targeted to the basolateral membrane in polarized epithelial cells and in hepatocytes, and to matrix vesicles in osteoblasts. In bile duct cells and cancer cells, located to the apical cytoplasmic side (By similarity). The proteolytically processed form is secreted. {ECO:0000250}.

Disease Associations

Note=Defects in Enpp1 are the cause of the tiptoe walking (ttw) phenotype. Ttw mice exhibit ossification of the spinal ligaments. {ECO:0000269PubMed:9662402}.

Tissue Specificity

Selectively expressed on the surface of antibody-secreting cells.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	1 [view]
Protein-Protein	0
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003676	nucleic acid binding
GO:0003824	catalytic activity
GO:0004528	phosphodiesterase I activity
GO:0004551	nucleotide diphosphatase activity
GO:0005044	scavenger receptor activity
GO:0005509	calcium ion binding
GO:0008270	zinc ion binding
GO:0016787	hydrolase activity
GO:0030247	polysaccharide binding
GO:0035529	NADH pyrophosphatase activity
GO:0046872	metal ion binding

Biological Process

GO:0006200	ATP catabolic process
GO:0006898	receptor-mediated endocytosis
GO:0006955	immune response
GO:0008152	metabolic process
GO:0030279	negative regulation of ossification
GO:0031214	biomineral tissue development
GO:0045599	negative regulation of fat cell differentiation
GO:0046849	bone remodeling
GO:0090305	nucleic acid phosphodiester bond hydrolysis

Cellular Component

GO:0005615	extracellular space
GO:0005886	plasma membrane
GO:0005887	integral component of plasma membrane
GO:0009986	cell surface
GO:0016021	integral component of membrane
GO:0016323	basolateral plasma membrane

Protein Structure and Domains

PDB ID

MGI:97370

InterPro

IPR001212 Somatomedin B domain
IPR001604 DNA/RNA non-specific endonuclease
IPR002591 Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase
IPR017850 Alkaline-phosphatase-like, core domain
IPR020436 Somatomedin B, chordata
IPR020821 Extracellular Endonuclease, subunit A

PFAM

PF01033
PF01223
PF01663

PRINTS

PR00022

PIRSF

SMART

SM00201
SM00892
SM00477

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt