InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Pld6

Summary

InnateDB Protein

IDBP-535147.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Pld6

Protein Name

phospholipase D family, member 6

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000115503

InnateDB Gene

IDBG-179431 (Pld6)

Protein Structure

UniProt Annotation

Function

Regulates mitochondrial shape through facilitating mitochondrial fusion. During spermatogenesis, plays a critical role in PIWI-interacting RNA (piRNA) biogenesis. piRNAs provide essential protection against the activity of mobile genetic elements. piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation. Has been proposed to act as a cardiolipin hydrolase to generate phosphatidic acid at mitochondrial surface (PubMed:21397848). Although it cannot be excluded that it can act as a phospholipase in some circumstances, it should be noted that cardiolipin hydrolase activity is either undetectable in vitro (PubMed:23064227 and PubMed:23064230), or very low. In addition, cardiolipin is almost exclusively found on the inner mitochondrial membrane, while PLD6 localizes to the outer mitochondrial membrane, facing the cytosol. Has been shown to be a backbone-non- specific, single strand-specific nuclease, cleaving either RNA or DNA substrates with similar affinity (PubMed:23064227 and PubMed:23064230). Produces 5' phosphate and 3' hydroxyl termini, suggesting it could directly participate in the processing of primary piRNA transcripts. {ECO:0000269PubMed:21397847, ECO:0000269PubMed:21397848, ECO:0000269PubMed:23064227, ECO:0000269PubMed:23064230}.

Subcellular Localization

Mitochondrion outer membrane {ECO:0000269PubMed:21397847}; Single-pass membrane protein {ECO:0000269PubMed:21397847}.

Disease Associations

Tissue Specificity

Predominantly expressed in testis (at protein level). Also expressed at high levels in growing ovary. {ECO:0000269PubMed:21397847}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003824	catalytic activity
GO:0004519	endonuclease activity
GO:0035755	cardiolipin hydrolase activity
GO:0042803	protein homodimerization activity
GO:0046872	metal ion binding

Biological Process

GO:0007126	meiotic nuclear division
GO:0007286	spermatid development
GO:0008053	mitochondrial fusion
GO:0008152	metabolic process
GO:0016042	lipid catabolic process
GO:0030719	P granule organization
GO:0034587	piRNA metabolic process
GO:0043046	DNA methylation involved in gamete generation
GO:0090305	nucleic acid phosphodiester bond hydrolysis