InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Brap

Summary

InnateDB Protein

IDBP-541685.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Brap

Protein Name

BRCA1 associated protein

Synonyms

3010002G07Rik; BRAP2; IMP;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000118574

InnateDB Gene

IDBG-197596 (Brap)

Protein Structure

UniProt Annotation

Function

Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto- polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport (By similarity). {ECO:0000250UniProtKB:Q7Z569}.

Subcellular Localization

Cytoplasm {ECO:0000250}.

Disease Associations

Tissue Specificity

Isoform 2 is highly expressed in testis, lower levels in brain, heart, lung, stomach, colon, uterus, liver and kidney. Isoform 1 is only expressed in the testis. Isoform 2 is predominant over isoform 1 in both fetal and adult testis. {ECO:0000269PubMed:14607334}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 9 experimentally validated interaction(s) in this database.
They are also associated with 26 interaction(s) predicted by orthology.

Experimentally validated
Total	9 [view]
Protein-Protein	9 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	26 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008139	nuclear localization sequence binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity
GO:0046872	metal ion binding

Biological Process

GO:0000165	MAPK cascade
GO:0007265	Ras protein signal transduction
GO:0009968	negative regulation of signal transduction
GO:0016567	protein ubiquitination

Cellular Component

GO:0000151	ubiquitin ligase complex
GO:0005737	cytoplasm

Protein Structure and Domains

PDB ID

MGI:1919649

InterPro

IPR001607 Zinc finger, UBP-type
IPR001841 Zinc finger, RING-type
IPR011011 Zinc finger, FYVE/PHD-type
IPR011422 BRCA1-associated 2
IPR018957 Zinc finger, C3HC4 RING-type

PFAM

PF02148
PF13639
PF14634
PF07576
PF00097

PRINTS

PIRSF

SMART

SM00290
SM00184

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q99MP8

PhosphoSite

PhosphoSite-Q99MP8

TrEMBL

UniProt Splice Variant

Entrez Gene

72399

UniGene

Mm.415675

RefSeq

MGI ID

MGI Symbol

Brap

OMIM

CCDS

HPRD

IMGT

EMBL

AF321920 AF321921 AK013885 AK034212 BC038490

GenPept

AAH38490 AAK28079 AAK28080 BAB29036 BAC28633