InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: WDR61

Summary

InnateDB Protein

IDBP-586431.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

WDR61

Protein Name

WD repeat domain 61

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000453801

InnateDB Gene

IDBG-24605 (WDR61)

Protein Structure

UniProt Annotation

Function

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C. {ECO:0000269PubMed:16024656, ECO:0000269PubMed:16307923, ECO:0000269PubMed:19952111, ECO:0000269PubMed:20178742}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:16024656}. Cytoplasm {ECO:0000305PubMed:16024656}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 49 experimentally validated interaction(s) in this database.
They are also associated with 3 interaction(s) predicted by orthology.

Experimentally validated
Total	49 [view]
Protein-Protein	49 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	3 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding

Biological Process

GO:0006351	transcription, DNA-templated
GO:0016055	Wnt signaling pathway
GO:0032968	positive regulation of transcription elongation from RNA polymerase II promoter
GO:0045638	negative regulation of myeloid cell differentiation
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0051571	positive regulation of histone H3-K4 methylation
GO:0080182	histone H3-K4 trimethylation
GO:2001162	positive regulation of histone H3-K79 methylation

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0016593	Cdc73/Paf1 complex
GO:0035327	transcriptionally active chromatin
GO:0055087	Ski complex

Protein Structure and Domains

PDB ID

InterPro

IPR001680 WD40 repeat
IPR013979 Translation initiation factor, beta propellor-like domain
IPR017986 WD40-repeat-containing domain
IPR020472 G-protein beta WD-40 repeat

PFAM

PF00400
PF08662

PRINTS

PR00320

PIRSF

SMART

SM00320

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt