InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RAB35

Summary

InnateDB Protein

IDBP-60136.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RAB35

Protein Name

RAB35, member RAS oncogene family

Synonyms

H-ray; RAB1C; RAY;

Species

Homo sapiens

Ensembl Protein

ENSP00000229340

InnateDB Gene

IDBG-60134 (RAB35)

Protein Structure

UniProt Annotation

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. {ECO:0000269PubMed:16950109, ECO:0000269PubMed:21951725}.

Subcellular Localization

Cell membrane {ECO:0000305}; Lipid-anchor {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Membrane, clathrin- coated pit. Cytoplasmic vesicle, clathrin-coated vesicle. Endosome. Melanosome. Note=Present on sorting endosomes and recycling endosome tubules. Tends to be enriched in PIP2-positive cell membrane domains. During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis. Identified in stage I to stage IV melanosomes.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 25 experimentally validated interaction(s) in this database.

Experimentally validated
Total	25 [view]
Protein-Protein	23 [view]
Protein-DNA	2 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003924	GTPase activity
GO:0005515	protein binding
GO:0005525	GTP binding
GO:0005546	phosphatidylinositol-4,5-bisphosphate binding
GO:0019003	GDP binding

Biological Process

GO:0000910	cytokinesis
GO:0006184	GTP catabolic process
GO:0006886	intracellular protein transport
GO:0006913	nucleocytoplasmic transport
GO:0007165	signal transduction
GO:0007264	small GTPase mediated signal transduction
GO:0008104	protein localization
GO:0015031	protein transport
GO:0016197	endosomal transport
GO:0019882	antigen processing and presentation
GO:0031175	neuron projection development
GO:0036010	protein localization to endosome
GO:1990090	cellular response to nerve growth factor stimulus

Cellular Component

GO:0005622	intracellular
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005886	plasma membrane
GO:0005905	coated pit
GO:0010008	endosome membrane
GO:0016020	membrane
GO:0031253	cell projection membrane
GO:0042470	melanosome
GO:0045171	intercellular bridge
GO:0045334	clathrin-coated endocytic vesicle
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR000795 Elongation factor, GTP-binding domain
IPR001806 Small GTPase superfamily
IPR002041 Ran GTPase
IPR003578 Small GTPase superfamily, Rho type
IPR003579 Small GTPase superfamily, Rab type
IPR005225 Small GTP-binding protein domain
IPR006689 Small GTPase superfamily, ARF/SAR type
IPR006762 Gtr1/RagA G protein
IPR013684 Mitochondrial Rho-like
IPR020849 Small GTPase superfamily, Ras type
IPR024156 Small GTPase superfamily, ARF type
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00009
PF00071
PF00025
PF04670
PF08477

PRINTS

PR00315
PR00449
PR00627
PR00328

PIRSF

SMART

SM00176
SM00174
SM00175
SM00173
SM00177

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt