InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Srsf1

Summary

InnateDB Protein

IDBP-620866.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Srsf1

Protein Name

serine/arginine-rich splicing factor 1

Synonyms

1110054N12Rik; 5730507C05Rik; 6330415C05Rik; AI482334; Asf; AW491331; Sf2; Sfrs1;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000133517

InnateDB Gene

IDBG-207877 (Srsf1)

Protein Structure

UniProt Annotation

Function

Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'- CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing (By similarity). Specifically regulates alternative splicing of cardiac isoforms of CAMK2D, LDB3/CYPHER and TNNT2/CTNT during heart remodeling at the juvenile to adult transition. The inappropriate accumulation of a neonatal and neuronal isoform of CAMKD2 in the adult heart results in aberrant calcium handling and defective excitation-contraction coupling in cardiomyocytes. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway. {ECO:0000250, ECO:0000269PubMed:15652482}.

Subcellular Localization

Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. Nucleus speckle {ECO:0000250}. Note=Shuttles between the nucleus and the cytoplasm. {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 42 experimentally validated interaction(s) in this database.
They are also associated with 127 interaction(s) predicted by orthology.

Experimentally validated
Total	42 [view]
Protein-Protein	22 [view]
Protein-DNA	17 [view]
Protein-RNA	3 [view]
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	127 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003723	RNA binding
GO:0003729	mRNA binding
GO:0005515	protein binding
GO:0050733	RS domain binding

Biological Process

GO:0000395	mRNA 5'-splice site recognition
GO:0001701	in utero embryonic development
GO:0008380	RNA splicing
GO:0051028	mRNA transport
GO:0060048	cardiac muscle contraction

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0016607	nuclear speck
GO:0035145	exon-exon junction complex
GO:0071013	catalytic step 2 spliceosome