InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: MED25

Summary

InnateDB Protein

IDBP-63578.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

MED25

Protein Name

mediator complex subunit 25

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000326767

InnateDB Gene

IDBG-63568 (MED25)

Protein Structure

UniProt Annotation

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription. {ECO:0000269PubMed:14657022, ECO:0000269PubMed:14983011, ECO:0000269PubMed:17641689}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:14657022}.

Disease Associations

Charcot-Marie-Tooth disease 2B2 (CMT2B2) [MIM:605589]: A recessive axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal degeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. {ECO:0000269PubMed:19290556}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Tissue Specificity

Ubiquitously expressed. Highest levels in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen. {ECO:0000269PubMed:14657022, ECO:0000269PubMed:14983011}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 62 experimentally validated interaction(s) in this database.
They are also associated with 3 interaction(s) predicted by orthology.

Experimentally validated
Total	62 [view]
Protein-Protein	61 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	3 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0008134	transcription factor binding
GO:0042974	retinoic acid receptor binding
GO:0046965	retinoid X receptor binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0006367	transcription initiation from RNA polymerase II promoter
GO:0008219	cell death
GO:0010467	gene expression
GO:0035563	positive regulation of chromatin binding
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0048147	negative regulation of fibroblast proliferation
GO:0071158	positive regulation of cell cycle arrest
GO:2001178	positive regulation of mediator complex assembly

Cellular Component

GO:0005654

nucleoplasm

Protein Structure and Domains

PDB ID

InterPro

IPR002035 von Willebrand factor, type A
IPR021394 Mediator complex, subunit Med25, PTOV activation and synapsin 2
IPR021397 Mediator complex, subunit Med25, synapsin 1
IPR021406 Mediator complex, subunit Med25, NR box
IPR021419 Mediator complex, subunit Med25, von Willebrand factor type A

PFAM

PF00092
PF11232
PF11235
PF11244
PF11265

PRINTS

PIRSF

SMART

SM00327

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q71SY5