InnateDB: Systems Biology of the Innate Immune Response

Bos taurus Protein: POLR3A

Summary

InnateDB Protein

IDBP-682369.2

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

POLR3A

Protein Name

DNA-directed RNA polymerase III subunit RPC1

Synonyms

Species

Bos taurus

Ensembl Protein

ENSBTAP00000017638

InnateDB Gene

IDBG-630873 (POLR3A)

Protein Structure

UniProt Annotation

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 23 interaction(s) predicted by orthology.

Predicted by orthology
Total	23 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0001056	RNA polymerase III activity
GO:0003677	DNA binding
GO:0003682	chromatin binding
GO:0003899	DNA-directed RNA polymerase activity
GO:0008270	zinc ion binding
GO:0016740	transferase activity
GO:0016779	nucleotidyltransferase activity
GO:0032549	ribonucleoside binding
GO:0046872	metal ion binding

Biological Process

GO:0002376	immune system process
GO:0006351	transcription, DNA-templated
GO:0006383	transcription from RNA polymerase III promoter
GO:0032728	positive regulation of interferon-beta production
GO:0045087	innate immune response
GO:0051607	defense response to virus

Cellular Component

GO:0005634	nucleus
GO:0005666	DNA-directed RNA polymerase III complex
GO:0016020	membrane

Protein Structure and Domains

PDB ID

InterPro

IPR000722 RNA polymerase, alpha subunit
IPR006592 RNA polymerase, N-terminal
IPR007066 RNA polymerase Rpb1, domain 3
IPR007080 RNA polymerase Rpb1, domain 1
IPR007081 RNA polymerase Rpb1, domain 5
IPR007083 RNA polymerase Rpb1, domain 4

PFAM

PF00623
PF04983
PF04997
PF04998
PF05000

PRINTS

PIRSF

SMART

SM00663

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

A4IF62