InnateDB: Systems Biology of the Innate Immune Response

Bos taurus Protein: CLP1

Summary

InnateDB Protein

IDBP-689914.2

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

CLP1

Protein Name

Polyribonucleotide 5'-hydroxyl-kinase Clp1

Synonyms

Species

Bos taurus

Ensembl Protein

ENSBTAP00000008507

InnateDB Gene

IDBG-637885 (CLP1)

Protein Structure

UniProt Annotation

Function

Polynucleotide kinase that can phosphorylate the 5'- hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus {ECO:0000255HAMAP-Rule:MF_03035}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 15 interaction(s) predicted by orthology.

Predicted by orthology
Total	15 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0005524	ATP binding
GO:0016301	kinase activity
GO:0016740	transferase activity
GO:0046404	ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity
GO:0051733	polydeoxyribonucleotide kinase activity
GO:0051736	ATP-dependent polyribonucleotide 5'-hydroxyl-kinase activity

Biological Process

GO:0006388	tRNA splicing, via endonucleolytic cleavage and ligation
GO:0006397	mRNA processing
GO:0008033	tRNA processing
GO:0016310	phosphorylation
GO:0021695	cerebellar cortex development
GO:0030423	targeting of mRNA for destruction involved in RNA interference
GO:0031124	mRNA 3'-end processing
GO:0035087	siRNA loading onto RISC involved in RNA interference

Cellular Component

GO:0000214	tRNA-intron endonuclease complex
GO:0005634	nucleus
GO:0005849	mRNA cleavage factor complex

Protein Structure and Domains

PDB ID

InterPro

IPR010655 Pre-mRNA cleavage complex II Clp1
IPR027417 P-loop containing nucleoside triphosphate hydrolase
IPR029007 MobB-type P-loop domain

PFAM

PF06807
PF03205

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

A2VE01

PhosphoSite

PhosphoSite-

TrEMBL

UniProt Splice Variant

Entrez Gene

506507

UniGene

Bt.15752

RefSeq

NP_001075182

HUGO

OMIM

CCDS

HPRD

IMGT

EMBL

BC133499

GenPept

AAI33500